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A simple and high-throughput method for xylem sap collection.
Biotechniques ( IF 2.7 ) Pub Date : 2019-09-18 , DOI: 10.2144/btn-2019-0046
Manman Kan 1 , Kiyoshi Yamazaki 1 , Toru Fujiwara 1 , Takehiro Kamiya 1, 2
Affiliation  

Xylem is the most important route for root-to-shoot translocation of water, nutrients and signaling molecules. Although a number of studies have been performed using xylem sap, its collection requires special equipment and is usually low throughput. Here, we developed a simple and high-throughput method for the collection of both medium- (<1 ml) and small- (<200 μl) volume xylem sap samples. Using a rice Cd transporter mutant, we demonstrated that our method allows for the effective evaluation of xylem sap Cd concentrations.

中文翻译:

一种简单,高通量的木质部汁液收集方法。

木质部是水,养分和信号分子从根到茎转移的最重要途径。尽管已经使用木质部树液进行了许多研究,但是其收集需要专用设备,并且通量通常较低。在这里,我们开发了一种简单且高通量的方法来收集中等体积(<1 ml)和小体积(<200μl)木质部树液样品。使用水稻镉转运蛋白突变体,我们证明了我们的方法可以有效评估木质部汁液镉的浓度。
更新日期:2020-08-21
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