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An idea to explore: Genotyping bull sperm to introduce basic molecular biology techniques in an animal science course
Biochemistry and Molecular Biology Education ( IF 1.4 ) Pub Date : 2019-08-30 , DOI: 10.1002/bmb.21297
Bert De Groef 1 , Sylvia V H Grommen 1
Affiliation  

The laboratory exercise described here aims to provide a relevant context for learning basic DNA techniques in an introductory animal science course at tertiary level. In two 4‐hr laboratory sessions, students assess the suitability of bulls for inclusion in a gene‐assisted selection program for A2 β‐casein by genotyping commercial bull sperm. Sperm cells are lysed to extract the genomic DNA, and PCR with primers for the β‐casein gene is performed. Using the principle of amplification‐created restriction sites, restriction digestion with TaqI can be used to distinguish between the A1 allele and the A2 allele of the gene. Cut PCR amplicons are separated by gel electrophoresis to evaluate the genotype of each bull. Students then write a diagnostic report with accompanying letter to their fictional client, explaining the DNA test, and interpreting the results. © 2019 International Union of Biochemistry and Molecular Biology, 47(6):708–711, 2019.

中文翻译:

一个探索的想法:基因分型公牛精子以在动物科学课程中介绍基本的分子生物学技术

此处描述的实验室练习旨在为在高等教育级别的介绍性动物科学课程中学习基本 DNA 技术提供相关背景。在两个 4 小时的实验室课程中,学生通过对商业公牛精子进行基因分型来评估公牛是否适合纳入 A2 β-酪蛋白的基因辅助选择计划。裂解精子细胞以提取基因组 DNA,并使用 β-酪蛋白基因的引物进行 PCR。利用扩增产生限制性位点的原理,TaqI 限制性消化可用于区分基因的 A1 等位基因和 A2 等位基因。通过凝胶电泳分离切割的 PCR 扩增子以评估每头公牛的基因型。然后学生写一份诊断报告,并附上一封信给他们虚构的客户,解释 DNA 测试,并解释结果。© 2019 国际生物化学与分子生物学联盟,47(6):708–711,2019。
更新日期:2019-08-30
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