Objective

This study mainly to explore the change of serum cytokines in wasp sting patients and the potential correlation between cytokines and acute kidney injury (AKI) due to wasp stings.

Methods

The levels of IL-2, IL-4, IL-6, IL-10, TNF-α, and IFN-γ in 33 wasp sting and 24 healthy people were measured by flow cytometry, the level of IL-17 was detected by enzyme-linked immunosorbent assay and the laboratory examination including inflammatory indicators, muscle enzyme markers, and renal function were detected by automatic biochemical analyzer, blood analyzer, and urine analyzer. The wasp sting patients were divided into AKI (n = 10) and non-AKI groups (n = 23). The correlation between the levels of serum cytokines and laboratory examination results was analyzed.

Results

The levels of IL-2, IL-6, IL-10, IFN-γ, and IL-17 were statistically increased in wasp sting patients compared with the controls (P < 0.05). IL-6, IL-10, and IL-17 levels were markedly increased in the AKI group compared with the non-AKI group (P < 0.05). Moreover, compared with non-AKI group, inflammatory markers and muscle enzyme markers were more abnormal in the AKI group. The positive rate of urinary occult blood in the AKI group was higher than that in the non-AKI group. The levels of IL-2, IL-4, IL-6, IFN-γ, and IL-17 correlated positively with white blood cell counts. The levels of IL-2, IL-4, IL-10, IFN-γ, and IL-17 correlated positively with the levels of serum creatinine. The levels of IL-2, IL-4, IL-10, IL-10, and IFN-γ correlated positively with the levels of C-reactive protein. The levels of IL-10, and IFN-γ correlated positively with urinary occult blood.

Conclusion

Elevated levels of cytokines in wasp sting patients might be involved in the development and progression of acute kidney injury.

中文翻译：

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细胞因子水平升高与黄蜂毒引起的急性肾损伤有关。

目的

这项研究主要探讨黄蜂叮咬患者血清细胞因子的变化以及细胞因子与黄蜂叮咬引起的急性肾损伤（AKI）之间的潜在相关性。

方法

流式细胞术检测33例黄蜂和24例健康人群IL-2，IL-4，IL-6，IL-10，TNF-α和IFN-γ的水平，并通过流式细胞术检测IL-17的水平。通过自动生化分析仪，血液分析仪和尿液分析仪检测酶联免疫吸附测定和实验室检查，包括炎症指标，肌肉酶标记和肾功能。将黄蜂st伤患者分为AKI组（n = 10）和非AKI组（n = 23）。分析血清细胞因子水平与实验室检查结果之间的相关性。

结果

与对照组相比，黄蜂st病患者的IL-2，IL-6，IL-10，IFN-γ和IL-17含量有统计学意义的升高（P <0.05）。与非AKI组相比，AKI组的IL-6，IL-10和IL-17水平显着升高（P<0.05）。此外，与非AKI组相比，AKI组的炎症标志物和肌肉酶标志物异常多。AKI组的尿潜血阳性率高于非AKI组。IL-2，IL-4，IL-6，IFN-γ和IL-17的水平与白细胞计数呈正相关。IL-2，IL-4，IL-10，IFN-γ和IL-17的水平与血清​​肌酐水平呈正相关。IL-2，IL-4，IL-10，IL-10和IFN-γ的水平与C反应蛋白的水平呈正相关。IL-10和IFN-γ的水平与尿潜血呈正相关。

结论

黄蜂患者的细胞因子水平升高可能与急性肾损伤的发生和发展有关。