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Murine mCLCA5 is expressed in granular layer keratinocytes of stratified epithelia.
Histochemistry and Cell Biology ( IF 2.3 ) Pub Date : 2009-12-11 , DOI: 10.1007/s00418-009-0667-0
Josephine Braun 1 , Melanie K Bothe , Lars Mundhenk , Carol L Beck , Achim D Gruber
Affiliation  

CLCA proteins represent a large family of proteins widely expressed in mammalian tissues with a unique expression pattern for each family member analyzed so far. However, their functions in normal and diseased tissues are poorly understood. Here, we present the cellular expression pattern of mCLCA5 in murine tissues using immunohistochemistry, confocal laser scanning microscopy and immune electron microscopy with specific antibodies and RT-qPCR following laser-capture microdissection. The mCLCA5 protein was localized to granular layer keratinocytes of virtually all stratified squamous epithelia of the body. Biochemical protein characterizations revealed that the amino-terminal cleavage product is fully secreted by the cell, while the carboxy-terminal cleavage product remains associated with the cell. The results imply that mCLCA5 may play a role in maturation and keratinization of squamous epithelial cells.

中文翻译:

鼠 mCLCA5 在分层上皮的颗粒层角质形成细胞中表达。

CLCA 蛋白代表了在哺乳动物组织中广泛表达的一大类蛋白质,迄今为止分析的每个家族成员都有独特的表达模式。然而,它们在正常和患病组织中的功能知之甚少。在这里,我们使用免疫组织化学、共聚焦激光扫描显微镜和具有特异性抗体的免疫电子显微镜以及激光捕获显微切割后的 RT-qPCR 来展示 mCLCA5 在鼠组织中的细胞表达模式。mCLCA5 蛋白定位于身体几乎所有分层鳞状上皮的颗粒层角质形成细胞。生化蛋白质表征表明氨基末端裂解产物完全由细胞分泌,而羧基末端裂解产物仍与细胞结合。
更新日期:2009-12-11
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