Abstract

The use of bisphenol S (BPS) as a substitute of Bisphenol A is increasing in several products and it can be found in different environmental and biological matrices. Its toxicity has been studied at different levels and one of BPS toxic mechanisms at high concentrations seems to be the induction of oxidative stress through the generation of reactive oxygen species (ROS). This study evaluates the ability of a curcuma and ginger (CG) mixture to exert an antioxidant effect on rat hepatocytes treated with BPS. The effects of the mixture were compared to those of a well-known antioxidant (Trolox). Three different BPS concentrations were used in order to verify ROS production. 70 µg/mL and 150 µg/mL of BPS generated a significant ROS increase (p < 0.01) as compared to control, while CG mixture was able to decrease this ROS production in hepatic cells, as compared to cells treated with 70 µg/ml of BPS (p < 0.01) restoring control levels. BPS 70 µg/mL was tested for total antioxidant capacity (TEAC), superoxide dismutase (SOD) and total thiols. TEAC and SOD significant decreased (p < 0.05 and p < 0.01, respectively) as compared to controls and CG mixture was able to restore control values. Given the widespread BPS use, results obtained in this study can be of high impact for the community, demonstrating the ability of a mixture of natural products to prevent BPS-induced oxidative stress.

摘要

双酚 S (BPS) 作为双酚 A 的替代品在多种产品中的使用正在增加，并且可以在不同的环境和生物基质中找到。已经在不同水平上研究了它的毒性，高浓度的 BPS 毒性机制之一似乎是通过产生活性氧 (ROS) 来诱导氧化应激。本研究评估姜黄和生姜 (CG) 混合物对用 BPS 处理的大鼠肝细胞发挥抗氧化作用的能力。将混合物的效果与众所周知的抗氧化剂 (Trolox) 的效果进行了比较。使用三种不同的 BPS 浓度来验证 ROS 的产生。70 µg/mL 和 150 µg/mL BPS 产生显着的 ROS 增加（p< 0.01) 与对照相比，而与用 70 µg/ml BPS ( p  < 0.01) 恢复对照水平 的细胞相比，CG 混合物能够降低肝细胞中的这种 ROS 产生。测试了 BPS 70 µg/mL 的总抗氧化能力 (TEAC)、超氧化物歧化酶 (SOD) 和总硫醇。与对照相比， TEAC 和 SOD 显着降低（分别为p  < 0.05 和p  < 0.01），并且 CG 混合物能够恢复对照值。鉴于 BPS 的广泛使用，本研究中获得的结果可能对社区产生重大影响，证明了天然产物混合物预防 BPS 诱导的氧化应激的能力。