Abstract

Portulacca oleracea L. has been used for treatment of different ailments. The aim of this study was to investigate the effectiveness and possible mechanism of action involved in the anti gastric ulcerogenic effect of Portulacca oleracea. Methanolic extract & subsequent fractions (100, 200 and 400 mg/kg) of Portulacca oleracea (P. oleracea) were administered orally to experimental rabbits one hour before oral administration of HCl/ethanol (40:60). Anti gastric ulcerogenic potential of P. oleracea was evaluated by assessment of gastric pH, pepsin, free acidity, ulcer index, mucus content and total acidity. For the investigation of possible mechanism of action malondialdehyde (MDA), histamine, and H + K + ATPase content were determined in the stomach homogenate. Histopathological study of stomach tissue was carried out by H&E dye. Ethyl acetate fraction (EAF) of P. oleracea was the most potent fraction among all fractions that exhibited efficient protection against acidified ethanol mediated gastric-ulcer. The ethyl acetate fraction (EAF) significantly increased the pH of gastric juice, while pepsin and histamine was observed to decrease significantly in comparison to acidified ethanol group (***p ≤ 0.001). The EAF showed moderately H + K + ATPase inhibitory activity. Moreover, it was also observed that EAF decreased the malondialdehyde (MDA) level in the stomach tissue homogenate showing antioxidant effect. Histopathological studies showed that among the tested fractions, EAF significantly prevented acidified ethanol induced gastric mucosal damage. These results showed that mechanism of anti gastric ulcerogenic potential of P. oleracea could be associated with the reduction in histamine level, H + K + ATPase inhibition and reduced MDA level.

摘要

Portulacca oleracea L. 已被用于治疗不同的疾病。本研究的目的是研究马齿苋抗胃溃疡作用的有效性和可能的​​作用机制。在口服 HCl/乙醇 (40:60) 前一小时，将马齿苋( P. oleracea ) 的甲醇提取物和后续部分 (100、200 和 400 mg/kg)口服给予实验兔。甘蓝的抗胃溃疡形成潜力通过评估胃的 pH 值、胃蛋白酶、游离酸度、溃疡指数、粘液含量和总酸度来评估。为了研究丙二醛 (MDA) 的可能作用机制，测定了胃匀浆中的组胺和 H + K + ATPase 含量。用H&E染料对胃组织进行组织病理学研究。甘蓝的乙酸乙酯馏分 (EAF)是所有馏分中最有效的馏分，对酸化乙醇介导的胃溃疡表现出有效的保护作用。与酸化乙醇组相比，乙酸乙酯组分 (EAF) 显着增加胃液的 pH 值，而观察到胃蛋白酶和组胺显着降低 (*** p ≤ 0.001)。EAF 显示出适度的 H + K + ATPase 抑制活性。此外，还观察到 EAF 降低了胃组织匀浆中的丙二醛 (MDA) 水平，显示出抗氧化作用。组织病理学研究表明，在测试的部分中，EAF 显着防止了酸化乙醇诱导的胃粘膜损伤。这些结果表明，甘蓝抗胃溃疡潜能的机制可能与降低组胺水平、抑制H + K + ATPase和降低MDA水平有关。