Ascomycota and basidiomycota fungi are prolific sources of biologically active natural products. Recent genomic data and bioinformatic analysis indicate that fungi possess a large number of biosynthetic gene clusters for bioactive natural products but more than 90% are silent. Heterologous expression in the filamentous fungi as hosts is one of the powerful tools to expression of the silent gene clusters. This review introduces recent studies on the total biosynthesis of representative family members via common platform intermediates, genome mining of novel di- and sesterterpenoids including detailed cyclization pathway, and development of expression host for basidiomycota genes with efficient genome editing method. In addition, this review will discuss the several strategies, for the generation of structural diversity, which are found through these studies.

中文翻译：

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异源宿主中真菌天然产物的生物合成机制的重构。

子囊菌和担子菌真菌是生物活性天然产物的多产来源。最近的基因组数据和生物信息学分析表明，真菌具有大量具有生物活性天然产物的生物合成基因簇，但90％以上是沉默的。作为宿主的丝状真菌中的异源表达是表达沉默基因簇的有力工具之一。这篇综述介绍了通过共同的平台中间体，代表家族成员的总生物合成的最新研究，新型双-和双萜类化合物的基因组挖掘（包括详细的环化途径），以及利用有效的基因组编辑方法开发了担子菌基因的表达宿主。此外，这篇评论将讨论几种结构多样性产生的策略，