Background: H+/K+ ATPase a protein present in the gastric parietal cells is a better target for the prevention and treatment of gastric ulcer. Plant flavonoids have been reported to elicit anti-ulcer activity by inhibiting the proton pump as well as by antioxidant defense mechanism.

Methods: Chloroform fraction of hydro-alcoholic extract of passion fruit was screened for proton pump inhibitory assay using goat parietal cell. In-silico computational docking studies were carried out using Glide program in order to validate the inhibitory action of selected constituents.

Results: The flavonoid rich fruit possess a promising radical scavenging activity against DPPH. 10.41μg/mL is sufficient to inhibit 50% of ATPase enzyme activity. A synergistic activity was also achieved by the fruit with sub-effective doses of lansoprazole. Fenton's oxidation induced by H2O2 was also blunted by the fruit extract.

Conclusion: The in-vitro and in-silico findings indicated that, passion fruit can be a good dietary supplement for the prevention and management of ulcer.

背景：存在于胃壁细胞中的 H+/K+ ATPase 是预防和治疗胃溃疡的更好靶点。据报道，植物黄酮类化合物通过抑制质子泵以及抗氧化防御机制来引发抗溃疡活性。

方法：使用山羊壁细胞筛选西番莲水醇提取物的氯仿级分用于质子泵抑制试验。使用 Glide 程序进行了计算机计算对接研究，以验证所选成分的抑制作用。

结果：富含类黄酮的果实对DPPH具有良好的自由基清除活性。10.41μg/mL 足以抑制 50% 的 ATPase 酶活性。与亚有效剂量的兰索拉唑的果实也实现了协同活性。由 H2O2 诱导的 Fenton 氧化也被水果提取物减弱。

结论：体外和计算机实验结果表明，百香果可以作为预防和管理溃疡的良好膳食补充剂。