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Improving anaerobic digestion via direct interspecies electron transfer requires development of suitable characterization methods.
Current Opinion in Biotechnology ( IF 7.7 ) Pub Date : 2019-06-04 , DOI: 10.1016/j.copbio.2019.03.018
Caroline Van Steendam 1 , Ilse Smets 2 , Steven Skerlos 3 , Lutgarde Raskin 3
Affiliation  

Recent anaerobic digestion studies commonly attribute performance improvements (e.g. increased methane production, enhanced process stability, reduced startup times) to direct interspecies electron transfer (DIET), even though only indirect evidence of DIET is available and DIET alone does not explain enhanced performance in many cases. This review evaluates methods believed to confirm the occurrence of DIET in anaerobic systems. 16S rRNA gene sequencing and meta-omics approaches are necessary to further DIET knowledge but are limited in their ability to confirm the occurrence of DIET. In situ use of cyclic voltammetry should be explored further, as well as microscopy and image analysis procedures to quantify stained cytochromes. Furthermore, linking interspecies distance, interspecies mixing, and cellular activity to a DIET-based electron transfer model is promising but needs further validation for anaerobic digestion systems. In short, a combination of methods is necessary to confirm the occurrence and expand our knowledge of DIET.

中文翻译:

通过直接种间电子转移改善厌氧消化需要开发合适的表征方法。

最近的厌氧消化研究通常将性能的提高(例如,甲烷产量的增加,工艺稳定性的提高,启动时间的减少)归因于直接种间电子转移(DIET),即使只有间接的DIET证据可用,而DIET本身并不能解释许多方面的增强性能。案件。这篇综述评估了被认为可以确认DIET在厌氧系统中的发生的方法。16S rRNA基因测序和元组学方法对于进一步了解DIET必不可少,但在确认DIET发生的能力方面受到限制。应进一步探索循环伏安法的原位使用,以及显微镜和图像分析程序以量化染色的细胞色素。此外,联系种间距离,种间混合,以及基于DIET的电子转移模型的细胞活性是有希望的,但需要进一步验证厌氧消化系统。简而言之,必须使用多种方法来确认发生并扩大我们对DIET的认识。
更新日期:2019-06-04
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