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Capsaicin And Genistein Override The Action Of Obestatin To Decrease Lipid Accumulation In 3T3-L1 Cells.
Cell Biochemistry and Biophysics ( IF 2.6 ) Pub Date : 2019-05-27 , DOI: 10.1007/s12013-019-00875-4
Musunuru Suneel Kumar Reddy 1, 2 , Uma V Manjappara 1, 2
Affiliation  

Satiety peptides convey information about short-term energy reserves in the gut to the hypothalamus and aid in regulation of appetite and food intake. Obestatin is one such gastro peptide that has been shown to upregulate glycerolipid metabolism and PPARγ signalling. Obestatin brings about moderate reduction in circulating and stored triglyceride levels and reduction in gain in body weight in mice. We wanted to test whether obestatin could be further potentiated by co-administration with nutraceuticals genistein and capsaicin that are well known to reduce triglyceride levels. Hence, we chose to administer the compounds individually and pair-wise with obestatin in 3T3-L1 cells at concentrations of 200 nM obestatin, 10 µM capsaicin and 100 µM genistein. When treated along with induction of differentiation, both capsaicin and genistein in combination with obestatin reduced triglyceride levels in 3T3-L1 cells by 25 and 20%, respectively, when accessed on day 14 after induction. The combined administrations were dominated by the effect of the nutraceuticals and showed the same effect as of capsaicin or genistein. Upregulation of Fatty acid synthase (Fasn) and Adipose triglyceride lipase (Atgl/Pnpla2) by obestatin were reversed by both capsaicin and genistein. However, their ability to upregulate Peroxisome proliferation activating receptor gamma (Pparγ), Hormone sensitive lipase (Hsl), Lipoprotein lipase (Lpl) were retained while upregulation of Uncoupling protein 1 (Ucp1) by capsaicin was unchanged upon co-administration. Over expression of the lipases and UCP1 in case of capsaicin could be resulting in net lowering of lipid accumulation in the cells.

中文翻译:

辣椒素和染料木黄酮可替代Obestatin降低3T3-L1细胞中脂质蓄积的作用。

饱腹感肽将有关肠道短期能量储备的信息传达至下丘脑,并有助于调节食欲和食物摄入量。肥胖抑制素是一种这样的胃肽,已被证明可上调甘油脂代谢和PPARγ信号传导。Obestatin可使小鼠的循环中和储存的甘油三酸酯水平适度降低,并减轻体重增加。我们想测试与已知能降低甘油三酯水平的保健品染料木素染料木素和辣椒素合用是否能进一步增强肥胖抑制素。因此,我们选择在3T3-L1细胞中以200 nM的抑素,10 µM的辣椒素和100 µM的染料木黄酮浓度分别将这些化合物与Obestatin配对使用。当与诱导分化一起治疗时,诱导后第14天,辣椒素和染料木黄酮与Obestatin联合使用均能使3T3-L1细胞中的甘油三酸酯水平分别降低25%和20%。联合给药以营养保健品的作用为主,并显示出与辣椒素或染料木黄酮相同的作用。脂肪酸合酶上调(辣椒素和染料木黄酮都可以逆转肥胖抑制的Fasn和脂肪甘油三酸酯脂酶(Atgl / Pnpla2)。然而,它们的能力上调过氧化物酶体增殖激活受体γ(PPAR γ),激素敏感脂肪酶(HSL),脂蛋白脂肪酶(LPL),而解偶联蛋白1(的上调被保留UCP1)由辣椒素是在共同施用不变。在辣椒素的情况下,脂肪酶和UCP1的过度表达可能会导致脂质在细胞中的蓄积净减少。
更新日期:2019-05-27
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