Recently, due to their promising applications in biomedicine, magnetic iron oxide nanoparticles (MPs) have become one of the research hotspots in the nanomedicine field. Since various synthetic modifications have been widely applied to these nanoparticles for better targeting behaviors, it is meaningful to apply the optimal magnetic field condition for each case. This will enable creating a safe and efficient drug targeting using different types of MPs. In the present study, we aimed to find out any changes of transepithelial transport of polysaccharide-coated MPs by applying the continuous or the pulsatile magnetic field condition. Our results with heparin-functionalized MPs indicate that the particle concentrations and the external magnetic field could influence the transepithelial permeability of the particles. In the presence of a continuously applied magnetic density, heparin-MPs at high concentrations, by forming magnetically-induced aggregation of particles over the cell surface layer, showed a lower cellular transport than those at low concentrations. Furthermore, the results from the quantitative chemical assays and imaging analyses showed that transepithelial transport of heparin-MPs (negatively charged) under the pulsatile magnetic field was higher than that under the continuous magnetic field (CP), whereas the starch-MPs (neutrally charged) showed a small difference in transepithelial transport or cell retention between pulsatile vs. continuous magnetic field conditions. Taken together, our results suggest that the external magnetic field should be differentially applied to control the cellular drug transport depending on the physicochemical properties of the surface chemistry of magnetic particles.

中文翻译：

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具有多糖表面涂层的磁性氧化铁颗粒的外部控制细胞运输。

近年来，由于其在生物医学中的应用前景广阔，磁性氧化铁纳米颗粒（MPs）已成为纳米医学领域的研究热点之一。由于各种合成修饰已广泛应用于这些纳米粒子以实现更好的靶向性能，因此针对每种情况应用最佳磁场条件是有意义的。这将使您能够使用不同类型的MP创建安全有效的靶向药物。在本研究中，我们旨在通过应用连续或脉动磁场条件来发现多糖包被的MPs跨上皮运输的任何变化。我们的肝素功能化MP的结果表明，颗粒浓度和外部磁场可能影响颗粒的跨上皮渗透性。在持续施加磁密度的情况下，高浓度的肝素-MPs通过在细胞表面层上形成磁性诱导的颗粒聚集而显示出比低浓度时更低的细胞转运。此外，定量化学分析和成像分析的结果表明，在脉动磁场下肝素-MPs（带负电荷）的跨上皮转运高于在连续磁场（CP）下的上皮转运，而淀粉-MPs（中性的） ）在搏动性和连续性磁场条件之间的跨上皮运输或细胞滞留性方面显示出很小的差异。在一起