SummaryEstablishment of cellular polarity is one of the key events during oocyte maturation. Inscuteable (Insc) has been identified as a key regulator of cell polarity during asymmetric division in Drosophila. However, the function of its evolutionarily conserved mammalian homologue, mInscuteable (mInsc), in mouse meiotic maturation is not clear. In this study, we investigated the roles of mInsc in mouse oocyte maturation. mInsc was detected at all stages of oocyte maturation. The protein level of mInsc was slightly higher at the germinal vesicle breakdown (GVBD) stage and remained constant during mouse oocyte maturation. The subcellular localization of mInsc overlapped with spindle microtubules. Disruption of microtubules and microfilaments caused changes in the localization of mInsc. Depletion or overexpression of mInsc significantly decreased the maturation rates of mouse oocytes. Depletion of mInsc significantly affected asymmetric division, spindle assembly, alignments of chromosomes and actin cap formation. Taken together, our results demonstrated that mInsc regulates meiotic spindle organization during mouse meiotic maturation.

中文翻译：

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mInscuteable 在小鼠卵母细胞减数分裂成熟过程中调节减数分裂纺锤体组织

摘要细胞极性的建立是卵母细胞成熟过程中的关键事件之一。Inscuteable (Insc) 已被确定为非对称分裂过程中细胞极性的关键调节因子果蝇. 然而，其进化上保守的哺乳动物同源物 mInscuteable (mInsc) 在小鼠减数分裂成熟中的功能尚不清楚。在这项研究中，我们研究了 mInsc 在小鼠卵母细胞成熟中的作用。在卵母细胞成熟的所有阶段都检测到 mInsc。mInsc 的蛋白质水平在生泡破裂 (GVBD) 阶段略高，并在小鼠卵母细胞成熟期间保持不变。mInsc 的亚细胞定位与纺锤体微管重叠。微管和微丝的破坏导致 mInsc 的定位发生变化。mInsc 的消耗或过表达显着降低了小鼠卵母细胞的成熟率。mInsc 的消耗显着影响不对称分裂、纺锤体组装、染色体排列和肌动蛋白帽形成。综合起来，