Biological dosimetry based on sulfhemoglobin (SHb), methemoglobin (MetHb), and carboxyhemoglobin (HbCO) levels was evaluated. SHb, MetHb and HbCO levels were estimated in erythrocytes of mice irradiated by γ rays from a 60Co source using the method of multi-component spectrophotometric analysis developed recently. In this method, absorption measurements of diluted aqueous Hb-solution were made at λ = 500, 569, 577 and 620 nm, and using the mathematical formulas based on multi-component spectrophotometric analysis and the mathematical Gaussian elimination method for matrix calculation, the concentrations of various Hb-derivatives and total Hb in mice blood were estimated. The dose range of γ rays was from 0.5 to 8 Gy and the dose rate was 0.5 Gy min-1. Among all Hb-derivatives, MetHb, SHb and HbCO demonstrated an unambiguous dose-dependent response. For SHb and MetHb, the detection limits were about 0.5 Gy and 1 Gy, respectively. After irradiation, high levels of MetHb, SHb and HbCO persisted for at least 10 days, and the maximal increase of MetHb, SHb and HbCO occurred up to 24 h following γ irradiation. The use of this "MetHb + SHb + HbCO"-derivatives-based absorbed dose relationship showed a high accuracy. It is concluded that simultaneous determination of MetHb, SHb and HbCO, by multi-component spectrophotometry provides a quick, simple, sensitive, accurate, stable and inexpensive biological indicator for the early assessment of the absorbed dose in mice.

中文翻译：

---

通过同时测定非活性血红蛋白衍生物作为生物剂量计，评估γ射线的吸收剂量。

评估了基于血红蛋白（SHb），高铁血红蛋白（MetHb）和羧基血红蛋白（HbCO）水平的生物剂量。使用最近开发的多组分分光光度分析方法，用60Co来源的γ射线辐照小鼠的红细胞中的SHb，MetHb和HbCO水平进行了估算。该方法在λ= 500、569、577和620 nm处测量了稀释的Hb溶液的吸收率，并使用基于多组分分光光度法的数学公式和用于矩阵计算的数学高斯消去法来计算浓度估算小鼠血液中各种Hb衍生物的含量和总Hb。γ射线的剂量范围是0.5至8Gy，剂量率是0.5Gy min-1。在所有Hb衍生物中，MetHb，SHb和HbCO表现出明确的剂量依赖性反应。对于SHb和MetHb，检出限分别约为0.5 Gy和1 Gy。辐照后，高水平的MetHb，SHb和HbCO持续至少10天，并且MetHb，SHb和HbCO的最大增加发生在γ辐照后的24小时内。基于这种“ MetHb + SHb + HbCO”-衍生物的吸收剂量关系显示出很高的准确性。结论是，通过多组分分光光度法同时测定MetHb，SHb和HbCO，可提供一种快速，简单，灵敏，准确，稳定和廉价的生物学指标，用于早期评估小鼠的吸收剂量。SHb和HbCO在γ辐照后长达24 h发生。基于这种“ MetHb + SHb + HbCO”-衍生物的吸收剂量关系显示出很高的准确性。结论是，通过多组分分光光度法同时测定MetHb，SHb和HbCO，可提供一种快速，简单，灵敏，准确，稳定和廉价的生物学指标，用于早期评估小鼠的吸收剂量。SHb和HbCO在γ辐照后长达24 h发生。基于这种“ MetHb + SHb + HbCO”-衍生物的吸收剂量关系显示出很高的准确性。结论是，通过多组分分光光度法同时测定MetHb，SHb和HbCO，可提供一种快速，简单，灵敏，准确，稳定和廉价的生物学指标，用于早期评估小鼠的吸收剂量。