Pentose sugars are increasingly being used in industrial applications of Saccharomyces cerevisiae. Although L-arabinose is a highlighted pentose that has been identified as next-generation biomass, arabinose fermentation has not yet undergone extensive development for industrial utilization. In this study, we integrated a heterologous fungal arabinose pathway with a deletion of PHO13 phosphatase gene. PHO13 deletion increased arabinose consumption rate and specific ethanol productivity under aerobic conditions and consequently depleted sedoheptulose by activation of the TAL1 gene. Global metabolite profiling indicated upregulation of the pentose phosphate pathway and downstream effects such as trehalose accumulation and downregulation of the TCA cycle. Our results suggest that engineering of PHO13 has ample potential for arabinose conversion to ethanol as an industrial source for biofuels.

中文翻译：

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删除PHO13可改善工程酿酒酵母中的好氧L-阿拉伯糖发酵。

戊糖被越来越多地用于酿酒酵母的工业应用中。尽管L-阿拉伯糖是已被鉴定为下一代生物质的突出的戊糖，但阿拉伯糖发酵尚未进行广泛的工业应用开发。在这项研究中，我们整合了带有PHO13磷酸酶基因缺失的异源真菌阿拉伯糖途径。在有氧条件下，PHO13缺失增加了阿拉伯糖的消耗速率和特定乙醇的生产率，因此通过激活TAL1基因减少了七庚糖。全球代谢物谱表明磷酸戊糖途径的上调和下游效应，例如海藻糖积累和TCA周期的下调。