The commercial transfection reagent Lipofectamine has been widely used for cytoplasmic delivery of nucleic acids and for cytosolic engagement with intracellular innate immune sensors to trigger type I interferon (IFN) production. However, the effect of Lipofectamine alone on type I IFN response has not been studied in detail. Here, we show that Lipofectamine induced type I IFN signaling in both RAW 264.7 macrophage-like cells and primary bone marrow-derived macrophages. Type I IFN induction was dependent on interferon regulatory factor (IRF)3 and IRF7 and partially required the toll/interleukin-1 receptor-domain-containing adapter-inducing interferon-β. In contrast, the transfection reagent Xfect did not activate type I IFN signaling. Our study highlights the potential confounding experimental interpretation when using Lipofectamine-based transfection for delivering intracellular ligands and provides important insights into lipid signaling in innate immune responses.

中文翻译：

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转染试剂Lipofectamine触发巨噬细胞中的I型干扰素信号传导激活。

商业转染试剂Lipofectamine已被广泛用于核酸的细胞质递送以及与细胞内先天免疫传感器的胞质结合以触发I型干扰素（IFN）的产生。但是，尚未单独研究脂转染胺对I型IFN反应的影响。在这里，我们表明，Lipofectamine诱导RAW 264.7巨噬细胞样细胞和原代骨髓来源的巨噬细胞中的I型IFN信号传导。I型IFN的诱导依赖于干扰素调节因子（IRF）3和IRF7，部分需要包含toll / interleukin-1受体域的衔接子诱导干扰素-β。相反，转染试剂Xfect没有激活I型IFN信号转导。