A cross-sectional study was conducted to determine the seroprevalence of Brucella spp. and Leptospira spp. and risk factors of exposure in cattle in three zones with varying land use types and wildlife–livestock interactions. Five villages were selected purposively; two in areas with intensive livestock–wildlife interactions (zone 1), another two in areas with moderate livestock–wildlife interactions (zone 2) and one in areas where wildlife–livestock interactions are rarer (zone 3). Sera samples were collected from 1170 cattle belonging to 390 herds in all the zones and tested for antibodies against Brucella abortus and Leptospira interrogans serovar hardjo using ELISA kits. Data on putative risk factors for seropositivity of these pathogens in cattle were collected using a questionnaire. The overall apparent animal-level seroprevalence of brucellosis and leptospirosis was, respectively, 36.9% (95% CI 34.1–39.8) and 23.5% (95% CI 21.1–26.0). Brucella spp. seroprevalence was higher in zone 1 than in zones 2 and 3 (χ² = 25.1, df = 2, P < 0.001). Zones 1 and 2 had significantly higher Leptospira spp. seroprevalence than zone 3 (χ² = 7.0, df = 2, P = 0.029). Results of multivariable analyses identified animal sex (female) and zones (high interface area) as significant predictors (P < 0.05) of animal-level seropositivity of Brucella spp. For Leptospira spp., important predictors of animal-level seropositivity were animal sex (female), zones (moderate interface area) and herds utilizing a communal grazing reserve. The seroprevalences of Brucella spp. and Leptospira spp. in cattle were higher in areas with moderate to high wildlife–livestock interactions than those with rare interactions.

中文翻译：

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肯尼亚野生动物-畜牧界面中的人畜共患病病原菌血清阳性率。

进行横断面研究以确定布鲁氏菌属的血清阳性率。和钩端螺旋体属。以及土地利用类型和野生动植物与牲畜相互作用各不相同的三个区域的牛暴露风险因素。目的是选择五个村庄；牲畜与野生动物互动密集的地区有两个（第1区），牲畜与野生动物互动中等的地区有第2个（第2区），野生动物与牲畜互动较少的地区有第三个（第3区）。从所有区域的390个牛群中的1170头牛中收集血清样本，并测试其抗流产布鲁氏菌和问号钩端螺旋体血清型硬jo的抗体使用ELISA试剂盒。使用问卷调查收集了这些病原体在牛中血清阳性的推定危险因素数据。布鲁氏菌病和钩端螺旋体病的总体表观动物血清阳性率分别为36.9％（95％CI 34.1-39.8）和23.5％（95％CI 21.1-26.0）。布鲁氏菌属。血清阳性率为在区域1高于区域2和3（χ ²  = 25.1，DF  = 2，P  <0.001）。1区和2区的钩端螺旋体物种明显较高。血清阳性率比区域3（χ ²  = 7.0，DF  = 2，P = 0.029）。多变量分析的结果确定动物性别（女性）和区域（高界面面积） 是布鲁氏菌属动物血清阳性的重要预测因子（P <0.05）。对于钩端螺旋体而言，动物水平血清阳性的重要预测指标是动物性别（雌性），区域（中度接触面积）和利用公共放牧储备的畜群。布鲁氏菌的血清阳性率。和钩端螺旋体属。与野生动物和牲畜互动程度中等至较高的地区，牛的数量要高于那些稀有动物。