Porcine Reproductive and Respiratory Syndrome (PRRS), an acute infectious disease caused by the porcine reproductive and respiratory syndrome virus (PRRSV), is one of the most devastating diseases affecting the global swine industry. In order to establish a multiplex real-time PCR method for the simultaneous detection of the classical PRRSV (C-PRRSV) strain, the highly pathogenic PRRSV (HP-PRRSV) strain and NADC30-like PRRSV (NL-PRRSV) strain, we designed specific primers and TaqMan fluorescent probes based on the Nsp2 target gene sequence of these three different PRRSV strains, and designed American-type PRRSV (PRRSV-U) special primers and probes based on the relatively conserved target gene sequence of ORF7. The method established in this study can quickly and accurately detect and differentiate three types of strains of clinical tissue samples, respectively. This method plays a key role in the rapid diagnosis and determination of PRRSV.

猪繁殖与呼吸综合症（PRRS）是由猪繁殖与呼吸综合症病毒（PRRSV）引起的一种急性传染病，是影响全球养猪业的最具破坏性的疾病之一。为了建立同时检测经典PRRSV（C-PRRSV）菌株，高致病性PRRSV（HP-PRRSV）菌株和类似NADC30的PRRSV（NL-PRRSV）菌株的多重实时PCR方法，我们设计了基于这三种不同PRRSV毒株的Nsp2靶基因序列的特异性引物和TaqMan荧光探针，并基于相对保守的ORF7靶基因序列设计了美国型PRRSV（PRRSV-U）特殊引物和探针。本研究中建立的方法可以快速，准确地检测和区分三种类型的临床组织样本菌株，分别。该方法在快速诊断和确定PRRSV中起着关键作用。