To enhance our ability to monitor poliovirus circulation and certify eradication, we evaluated the performance of the bag-mediated filtration system (BMFS) against the two-phase separation (TPS) method for concentrating wastewater samples for poliovirus detection. Sequential samples were collected at two sites in Mexico; one L was collected by grab and ~ 5 L were collected and filtered in situ with the BMFS. In the laboratory, 500 mL collected by grab were concentrated using TPS and the sample contained in the filter of the BMFS was eluted without secondary concentration. Concentrates were tested for the presence of poliovirus and non-poliovirus enterovirus (NPEV) using Global Poliovirus Laboratory Network standard procedures. Between February 16, 2016, and April 18, 2017, 125 pairs of samples were obtained. Collectors spent an average (± standard deviation) of 4.3 ± 2.2 min collecting the TPS sample versus 73.5 ± 30.5 min collecting and filtering the BMFS sample. Laboratory processing required an estimated 5 h for concentration by TPS and 3.5 h for elution. Sabin 1 poliovirus was detected in 37 [30%] samples with the TPS versus 24 [19%] samples with the BMFS (McNemar’s mid p value = 0.004). Sabin 3 poliovirus was detected in 59 [47%] versus 49 (39%) samples (p = 0.043), and NPEV was detected in 67 [54%] versus 40 [32%] samples (p < 0.001). The BMFS method without secondary concentration did not perform as well as the TPS method for detecting Sabin poliovirus and NPEV. Further studies are needed to guide the selection of cost-effective environmental surveillance methods for the polio endgame.

中文翻译：

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2016-2017 年墨西哥废水样品中脊灰病毒两种检测方法的现场表现。

为了增强监测脊髓灰质炎病毒循环和证明消灭的能力，我们针对浓缩废水样本进行脊髓灰质炎病毒检测的两相分离 (TPS) 方法评估了袋介导过滤系统 (BMFS) 的性能。在墨西哥的两个地点连续采集样本；通过抓取收集 1 L，并使用 BMFS 收集和原位过滤约 5 L。在实验室中，通过抓取收集的 500 mL 使用 TPS 进行浓缩，并且将 BMFS 过滤器中包含的样品洗脱，无需二次浓缩。使用全球脊髓灰质炎病毒实验室网络标准程序对浓缩物进行了脊髓灰质炎病毒和非脊髓灰质炎病毒肠道病毒 (NPEV) 的检测。2016年2月16日至2017年4月18日期间，共获取样本125对。收集者平均（±标准差）花费 4.3 ± 2.2 分钟收集 TPS 样本，而收集和过滤 BMFS 样本则花费 73.5 ± 30.5 分钟。实验室处理预计需要 5 小时进行 TPS 浓缩，3.5 小时进行洗脱。使用 TPS 在 37 个 [30%] 个样本中检测到 Sabin 1 脊髓灰质炎病毒，而使用 BMFS 在 24 个 [19%] 个样本中检测到 Sabin 1 脊髓灰质炎病毒（McNemar 的中值p值 = 0.004）。在 59 个 [47%] 样本与 49 个 (39%) 个样本中检测到 Sabin 3 脊髓灰质炎病毒 ( p  = 0.043)，在 67 个 [54%] 个样本与 40 个 [32%] 个样本中检测到 NPEV ( p  < 0.001)。没有二次浓缩的 BMFS 方法在检测萨宾脊髓灰质炎病毒和 NPEV 方面的表现不如 TPS 方法。需要进一步的研究来指导选择具有成本效益的环境监测方法来应对脊髓灰质炎的最终结果。