α-Amylases are used in various biotechnological processes including the textile, paper, food, biofuels, detergents and pharmaceutical industries. In this study, a novel gene encoding α-amylase was cloned from marine bacterium Salinispora arenicola CNP193 and the protein was expressed in Escherichia coli. The α-amylase gene from S. arenicola CNP193 had a length of 1839 bp and encoded a α-amylase with an estimated molecular mass of 74 kDa. The optimum temperature and pH for the recombinant α-amylase was 50 °C and 7 respectively. Na⁺, K⁺ and Ca²⁺ increased the activity of the recombinant α-amylase whereas the enzyme was inhibited by Cu²⁺, Zn²⁺, Hg²⁺, Pb²⁺, Fe³⁺ and Mn²⁺. Thin layer chromatography results confirmed that monosaccharide, disaccharide and maltotriose are the hydrolysis products. The results of our study suggest that this enzyme has considerable potential in industrial applications.

中文翻译：

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沙门氏菌CNP193的新型α-淀粉酶的克隆，表达和鉴定。

α-淀粉酶可用于各种生物技术过程，包括纺织，造纸，食品，生物燃料，清洁剂和制药工业。在这项研究中，从海洋细菌沙门氏菌（Salinispora arenicola） CNP193克隆了一个编码α-淀粉酶的新基因，该蛋白在大肠杆菌中表达。来自沙门氏菌CNP193的α-淀粉酶基因的长度为1839bp，并且编码α-淀粉酶，其估计分子量为74kDa。重组α-淀粉酶的最佳温度和pH分别为50℃和7。Na ⁺，K ⁺和Ca ²⁺增加了重组α-淀粉酶的活性，而该酶却受到Cu 2 ⁺，Zn ^2+的抑制。，Hg 2 ⁺，Pb 2 ⁺，Fe ³⁺和Mn ²⁺。薄层色谱结果证实单糖，二糖和麦芽三糖是水解产物。我们的研究结果表明，这种酶在工业应用中具有相当大的潜力。