Mango (Mangifera indica L.) is an economically important fruit. However, the marketability of mango is affected by the perishable nature and short shelf-life of the fruit. Therefore, a better understanding of the mango ripening process is of great importance towards extending its postharvest shelf life. Proteomics is a powerful tool that can be used to elucidate the complex ripening process at the cellular and molecular levels. This study utilized 2-dimensional gel electrophoresis (2D-GE) coupled with MALDI-TOF/TOF to identify differentially abundant proteins during the ripening process of the two varieties of tropical mango, Mangifera indica cv. ‘Chokanan’ and Mangifera indica cv ‘Golden Phoenix’. The comparative analysis between the ripe and unripe stages of mango fruit mesocarp revealed that the differentially abundant proteins identified could be grouped into the three categories namely, ethylene synthesis and aromatic volatiles, cell wall degradation and stress–response proteins. There was an additional category for differential proteins identified from the ‘Chokanan’ variety namely, energy and carbohydrate metabolism. However, of all the differential proteins identified, only methionine gamma-lyase was found in both ‘Chokanan’ and ‘Golden Phoenix’ varieties. Six differential proteins were selected from each variety for validation by analysing their respective transcript expression using reverse transcription-quantitative PCR (RT-qPCR). The results revealed that two genes namely, glutathione S-transferase (GST) and alpha-1,4 glucan phosphorylase (AGP) were found to express in concordant with protein abundant. The findings will provide an insight into the fruit ripening process of different varieties of mango fruits, which is important for postharvest management.

中文翻译：

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两种热带芒果（Mangifera indica）果实成熟过程的比较蛋白质组学分析。

芒果（Mangifera indica L.）是一种经济上重要的水果。但是，芒果的适销性受水果的易腐性和较短的货架期影响。因此，更好地了解芒果成熟过程对于延长其收获后的货架寿命至关重要。蛋白质组学是一种功能强大的工具，可用于阐明细胞和分子水平的复杂成熟过程。这项研究利用二维凝胶电泳（2D-GE）结合MALDI-TOF / TOF来鉴定热带芒果两个品种Mangifera indica cv的成熟过程中差异丰富的蛋白质。'Chokanan'和印度芒果简历“金凤凰”。对芒果果实中果皮成熟和未成熟阶段的比较分析表明，鉴定出的差异丰富的蛋白质可分为三类，即乙烯合成和芳香族挥发物，细胞壁降解和应激反应蛋白质。从“ Chokanan”品种中鉴定出的差异蛋白还有一个类别，即能量和碳水化合物代谢。但是，在所有鉴定出的差异蛋白中，“ Chokanan”和“ Golden Phoenix”品种中均仅发现蛋氨酸γ-裂合酶。通过使用逆转录定量PCR（RT-qPCR）分析其各自的转录表达，从每个品种中选择六个差异蛋白进行验证。结果揭示了两个基因，即 发现谷胱甘肽S-转移酶（GST）和α-1,4葡聚糖磷酸化酶（AGP）表达与丰富的蛋白质一致。这些发现将为深入了解芒果品种不同的果实成熟过程提供参考，这对于收获后的管理非常重要。