MicroRNA (miR)-204 is known to be associated with several different diseases. Polycystic ovary syndrome (PCOS) has the highest incidence rate among the endocrine disorders in females between the ages of 18 and 44. We aimed to illustrate the miR-204 function in PCOS. MiR-204 expression levels in tissue and cell were examined through RT-qPCR. Colony formation assay and MTT assay were applied to detect the cell viability. Flow cytometry was employed to examine the apoptosis and cell cycle in cells. RNA binding protein immunoprecipitation assay and luciferase reporter assay were provided to demonstrate the direct interaction between translationally controlled tumor protein (TPT1) and miR-204. The expression of miR-204 was declined in KGN cells and ovarian cortex tissues of PCOS patients. MiR-204 enhanced the colony formation capacity and cell proliferation in KGN cells. Cell cycle and apoptosis were also influenced by miR-204. Since miR-204 has direct interaction with TPT1, TPT1 overexpression suppressed the miR-204-induced apoptosis and cell cycle alteration in KGN cells. MiR-204 inhibits the cell viability and induces apoptosis and cell cycle arrest by directly interacting with TPT1, indicating a role of miR-204 to be a potential target in the PCOS patients.

中文翻译：

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MiR-204 通过靶向多囊卵巢综合征中的 TPT1 抑制细胞增殖并促进卵巢颗粒细胞凋亡。

已知微小 RNA (miR)-204 与几种不同的疾病有关。多囊卵巢综合征 (PCOS) 在 18 至 44 岁女性的内分泌疾病中发病率最高。我们旨在阐明 miR-204 在 PCOS 中的功能。通过 RT-qPCR 检查组织和细胞中的 MiR-204 表达水平。应用集落形成测定和MTT测定来检测细胞活力。流式细胞术用于检测细胞的凋亡和细胞周期。提供了 RNA 结合蛋白免疫沉淀测定和荧光素酶报告测定，以证明翻译控制肿瘤蛋白 (TPT1) 和 miR-204 之间的直接相互作用。miR-204的表达在PCOS患者的KGN细胞和卵巢皮质组织中下降。MiR-204 增强了 KGN 细胞的集落形成能力和细胞增殖。细胞周期和细胞凋亡也受 miR-204 的影响。由于 miR-204 与 TPT1 直接相互作用，TPT1 过表达抑制了 miR-204 诱导的 KGN 细胞凋亡和细胞周期改变。MiR-204 通过直接与 TPT1 相互作用抑制细胞活力并诱导细胞凋亡和细胞周期停滞，表明 miR-204 的作用是 PCOS 患者的潜在靶点。