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Inactivation of Adenovirus in Water by Natural and Synthetic Compounds.
Food and Environmental Virology ( IF 3.4 ) Pub Date : 2019-02-04 , DOI: 10.1007/s12560-019-09370-8
Lucas Ariel Totaro Garcia 1 , Laurita Boff 1 , Célia Regina Monte Barardi 1 , Markus Nagl 2
Affiliation  

Millions of people use contaminated water sources for direct consumption. Chlorine is the most widely disinfection product but can produce toxic by-products. In this context, natural and synthetic compounds can be an alternative to water disinfection. Therefore, the aim of this study was to assess the inactivation of human adenovirus by N-chlorotaurine (NCT), bromamine-T (BAT) and Grape seed extract (GSE) in water. Distilled water artificially contaminated with recombinant human adenovirus type 5 (rAdV-GFP) was treated with different concentrations of each compound for up to 120 min, and viral infectivity was assessed by fluorescence microscopy. The decrease in activity of the compounds in the presence of organic matter was evaluated in water supplemented with peptone. As results, NCT and GSE inactivated approximately 2.5 log10 of adenovirus after 120 min. With BAT, more than 4.0 log10 decrease was observed within 10 min. The oxidative activity of 1% BAT decreased by 50% in 0.5% peptone within a few minutes, while the reduction was only 30% for 1% NCT in 5% peptone after 60 min. Organic matter had no effect on the activity of GSE. Moreover, the minimal concentration of BAT and GSE to kill viruses was lower than that known to kill human cells. It was concluded that the three compounds have potential to be used for water disinfection for drinking or reuse purposes.

中文翻译:

天然和合成化合物灭活水中的腺病毒。

数百万人使用受污染的水源直接消费。氯是最广泛的消毒产品,但会产生有毒的副产品。在这种情况下,天然和合成化合物可以替代水消毒。因此,本研究的目的是评估水中N-氯牛磺酸(NCT),溴胺T(BAT)和葡萄籽提取物(GSE)对人腺病毒的灭活作用。用不同浓度的每种化合物处理被5型重组人腺病毒(rAdV-GFP)人工污染的蒸馏水长达120分钟,并通过荧光显微镜评估病毒的感染性。在补充蛋白ept的水中评估了有机物存在下化合物活性的降低。结果,NCT和GSE灭活了约2.5 log120分钟后,感染10支腺病毒。使用BAT时,在10分钟内观察到超过4.0 log 10的下降。在60分钟后,0.5%蛋白p中1%BAT的氧化活性降低了50%,而5%蛋白ept中1%NCT中的氧化活性仅降低了30%。有机物对GSE的活性没有影响。此外,杀死病毒的BAT和GSE的最低浓度低于杀死人类细胞的最低浓度。得出的结论是,这三种化合物具有用于饮用水消毒或再利用目的的水消毒潜力。
更新日期:2019-02-04
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