Necrosis-inducing anticancer drugs enhance high-mobility group box 1 (HMGB1) release during cell necrosis, and HMGB1-induced autophagy in skeletal muscle induces muscle atrophy. We evaluated the efficacy of magnetic hyperthermia therapy (MHT) using a low-energy magnetic field and self-controlled heating elements in tumor treatment. MHT-induced apoptosis by heating mouse subcutaneous tumors at 43°C using a heat-controlling iron-aluminum (Fe-Al) milling alloy. In contrast, MHT using Fe line-induced necrosis by heating to approximately 100°C. Furthermore, MHT with Fe-Al milling alloy reduced stemness. In hyperthermia using age line or Fe-Al milling alloy, both of them provided histological degeneration in skeletal muscle; however, qualitative differences were observed. MHT using Fe-line induced pronounced autophagy, decrease of myosin heavy chain content, and increase in serum HMGB1. In contrast, MHT using Fe-Al milling alloy induced heat shock protein 90 but no autophagy and decreased serum HMGB1. Therefore, MHT using Fe-Al milling alloy might be a good method for local treatment of tumors to reduce skeletal muscle atrophy.

中文翻译：

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使用由铁铝铣削合金组成的自控加热元件的磁热疗在保留骨骼肌的同时诱导癌细胞凋亡

坏死诱导抗癌药物在细胞坏死过程中增强高迁移率族框 1 (HMGB1) 的释放，HMGB1 诱导的骨骼肌自噬诱导肌肉萎缩。我们评估了使用低能磁场和自控加热元件的磁热疗 (MHT) 在肿瘤治疗中的疗效。通过使用热控铁铝 (Fe-Al) 铣削合金在 43°C 下加热小鼠皮下肿瘤，MHT 诱导细胞凋亡。相比之下，MHT 通过加热到大约 100°C 使用 Fe 线诱导坏死。此外，带有 Fe-Al 铣削合金的 MHT 降低了干度。在使用年龄线或铁铝铣削合金的热疗中，它们都提供了骨骼肌的组织学变性；然而，观察到了质的差异。MHT 使用 Fe-line 诱导明显的自噬，肌球蛋白重链含量减少，血清 HMGB1 增加。相比之下，使用 Fe-Al 研磨合金的 MHT 诱导热休克蛋白 90 但没有自噬并降低血清 HMGB1。因此，使用Fe-Al铣削合金的MHT可能是局部治疗肿瘤以减少骨骼肌萎缩的好方法。