Background: Laccases (Lacs) are used potentially in industrial and biotechnological applications such as decolorization of dyes, degradation of industrial effluents, delignification, etc. thanks to their large varieties of substrate specificities and excellent catalytic efficiencies. The efficient utilizations of Lacs in these applications mostly depend on the identifying their biochemical properties.

Objective: The goal of this research is to investigate the purification, biochemical characterization and decolorization efficiencies of Lacs.

Methods: Pleurotus eryngii was incubated on peach (PC) and cherry (CC) wastes under optimized solid state fermentation conditions. Then, the enzymes extracts were purified by ammonium sulfate precipitation, anion exchange chromatography, gel filtration, respectively. Lacs fractions were subjected to electrophoretic analyses as well as their structural and kinetic characteristics. Also, the effects of selected chemical agents on purified Lacs activities and determination of decolorization efficiencies were studied.

Results: As the results of purification processes of Lacs from both cultures, 3.94-fold purification was obtained for PC, while it was 5.34 for CC. The electrophoretic results of purified Lacs illustrated the single bands of protein (30±1 kDa) in accordance with the results after gel filtration. The Km values of Lacs from PC and CC were respectively detected as 1.1381 and 0.329 mM for ABTS. The selected agents partially/completely inhibited Lac activities. The highest decolorization efficiencies of purified Lacs from PC and CC were separately obtained as 53 and 11.8%.

Conclusion: The results clearly indicated that the performances of Lacs from both cultures in decolorization application are different from each other depending their activities, biochemical and kinetic characteristics.

背景：漆酶（Lacs）潜在地用于工业和生物技术应用，例如染料的脱色，工业废水的降解，脱木素等，这是由于它们具有多种底物特异性和出色的催化效率。这些应用中Lacs的有效利用主要取决于鉴定其生化特性。

目的：本研究的目的是研究Lacs的纯化，生化特性和脱色效率。

方法：在优化的固态发酵条件下，将杏鲍菇侧柏在桃（PC）和樱桃（CC）废物上孵育。然后，分别通过硫酸铵沉淀，阴离子交换色谱，凝胶过滤纯化酶提取物。对Lacs馏分及其结构和动力学特性进行电泳分析。此外，还研究了所选化学试剂对纯化的Lacs活性的影响以及脱色效率的测定。

结果：从两种培养物中纯化Lacs的结果表明，PC的纯化率为3.94倍，CC的纯化率为5.34倍。根据凝胶过滤后的结果，纯化的Lacs的电泳结果说明了蛋白质的单条带（30±1 kDa）。对于ABTS，来自PC和CC的Lacs的Km值分别检测为1.1381和0.329 mM。所选药剂部分/完全抑制了Lac活性。从PC和CC纯化的Lacs的最高脱色效率分别为53和11.8％。

结论：结果清楚地表明，两种文化的Lacs在脱色应用中的性能各不相同，这取决于它们的活性，生化和动力学特性。