We developed a biotin-streptavidin-based sandwich ELISA for the sensitive and specific detection of Yersinia pestis. In this assay, the F1 capsular protein and Y. pestis were captured by anti-F1 mouse monoclonal antibody followed by detection with biotinylated-anti-F1 rabbit polyclonal antibody and HRP-conjugated streptavidin. The developed F1 ELISA could detect not only the F1 protein up to 29 and 17 pg/ml but also Y. pestis up to 177.8 and 129.2 CFU/ml in PBS buffer and human serum, respectively. In addition, the F1 ELISA did not show any cross-reactivity with various proteins and bacterial strains.

中文翻译：

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开发用于灵敏检测鼠疫耶尔森氏菌的双抗体夹心ELISA。

我们开发了一种基于生物素-链霉亲和素的夹心ELISA，用于鼠疫耶尔森氏菌的灵敏和特异检测。在该测定中，F1荚膜蛋白和鼠疫耶尔森氏菌被抗F1小鼠单克隆抗体捕获，然后用生物素化的抗F1兔多克隆抗体和结合HRP的链霉亲和素进行检测。所开发的F1 ELISA不仅可以检测PBS缓冲液和人血清中高达29和17 pg / ml的F1蛋白，而且还可以分别检测高达177.8和129.2 CFU / ml的鼠疫耶尔森氏菌。此外，F1 ELISA与各种蛋白质和细菌菌株均无交叉反应。