Little is known about endophytic microbes in cold climate plants and how their communities are formed. We compared culturable putative endophytic bacteria and fungi in the ecologically important circumpolar grass, Deschampsia flexuosa growing in two successional stages of subarctic sand dune (68°29′N). Sequence analyses of partial 16S rRNA and internal transcribed spacer (ITS) sequences of culturable endophytes showed that diverse bacteria and fungi inhabit different tissues of D. flexuosa. A total of 178 bacterial isolates representing seven taxonomic divisions, Alpha, Beta and Gammaproteobacteria, Actinobacteria, Bacteroidetes, Firmicutes and Acidobacteria, and 30 fungal isolates representing the phylum Ascomycota were identified. Several endophytes were affiliated with specific plant tissues or successional stages. This first report of bacterial endophytes in D. flexuosa revealed that the genus Pseudomonas is tightly associated with D. flexuosa, and encompassed 39% of the bacterial isolates, and 58% of seed isolates. Based on 16S rRNA and ITS sequence data, most of the D. flexuosa endophytes were closely related to microbes from other cold environments. The majority of seed endophytic bacterial isolates were able to solubilize organic form of phosphate suggesting that these endophytes could play a role in resource mobilization in germinating seeds in nutrient‐poor habitat.

中文翻译：

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北极亚陆内陆一次演替中的极地草中的可培养的内生微生物群落，即Deschampsia flexuosa具有特定的生境和生长期。

对于寒冷气候植物中的内生微生物及其群落形成方式知之甚少。我们比较了在生态上重要的极地草，弯曲的D eschampsia flexuosa在两个连续的亚沙丘沙丘（68°29'N）阶段生长的可培养的内生细菌和真菌。可培养内生菌的部分16S rRNA和内部转录间隔区（ITS）序列的序列分析表明，不同的细菌和真菌栖息在D的不同组织中。flexuosa。共有178个细菌分离株，分别代表7个分类学领域，即Alpha，Beta和Gammaproteobacteria，放线菌，拟杆菌，确认了硬毛菌和酸杆菌，并鉴定了代表子囊门的30种真菌分离株。几种内生菌与特定的植物组织或演替阶段有关。这是D.中细菌内生菌的首次报道。flexuosa显示，该属P假单胞菌紧密关联d 。flexuosa，并且所包括的细菌分离株的39％，和种子分离物的58％。基于16S rRNA和ITS序列数据，大多数的d 。柔术内生菌与来自其他寒冷环境的微生物密切相关。大多数种子内生细菌分离株能够溶解有机形式的磷酸盐，这表明这些内生菌可以在营养缺乏的生境中发芽种子时在资源动员中发挥作用。