Cotton production is widely effected by Cotton Leaf Curl Virus (CLCuV) in world posing serious losses to cotton yield.The CRT genes from CLCuV resistant G. arboreum and CLCuV susceptible G. hirsutum were cloned and sequenced to know the differences of protein composition in both species. Molecular techniques were used to isolate full length putative biotic stress resistance genes from G. arboreum besides the analysis of identified novel genes in model plant tobacco (Nicotiana tabacum) for resistance to cotton leaf curl disease complex. It was found that transgenic plants over expressing Hydroperoxidelyase (HPL) genes exhibited higher enzyme activity than wild type. In addition the genome sequence information was used for the purpose of gene isolation. Even for the enhanced expression of Calreticulin (CRT), AOS and HPL in G. hirsutum, it still showed susceptibility against CLCuV suggesting alternative genes and pathways involved for the expression of resistance.

中文翻译：

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从棉花（棉）中分离生物逆境抗性基因，并在模型植物烟草（烟草）中对棉叶卷曲病复合物的抗性进行分析。

在世界范围内，棉花叶片卷曲病毒（CLCuV）对棉花的生产造成了广泛影响，严重损害了棉花的产量。克隆了耐CLCuV的植物棉和易感黄瓜的CRT基因的CRT基因并进行了测序，以了解两者的蛋白质组成差异种类。除了分析模型植物烟草中对烟叶卷曲病复合物具有抗性的新基因分析之外，还使用分子技术从植物假单胞菌中分离了全长推定的生物胁迫抗性基因。发现过表达氢过氧化物酶（HPL）基因的转基因植物显示出比野生型更高的酶活性。另外，基因组序列信息用于基因分离的目的。即使为了增强钙调蛋白（CRT），AOS和HPL在陆地棉中的表达，