The avulsion of nerve roots of the brachial plexus that is commonly seen in motorcycle accidents is a type of neuropathy due to deafferentation. This type of pain is clinically challenging since therapeutical protocols fail or have severe side effects. Thus, it is proposed to evaluate the antinociceptive activity of the recombinant CTK 01512-2 peptide that is derived from the venom of the Phoneutria nigriventer spider, as a future new therapeutical option. The neuropathic pain was surgically induced by avulsion of the upper brachial plexus trunk in groups of male Wistar rats and after 17 days, they were treated intrathecally with morphine, ziconotide, and CTK 01512-2. Behavioral tests were performed to evaluate mechanical and thermal hyperalgesia, cold allodynia, the functional activity of the front paw, and exploratory locomotion after the treatments. The peripheral blood samples were collected 6 h after the treatments and a comet assay was performed. The spinal cord was removed for the lipoperoxidation dosing of the membranes. The cerebrospinal fluid was analyzed for the dosage of glutamate. The recombinant peptide showed an antinociceptive effect when compared to the other drugs, without affecting the locomotor activity of the animals. Mechanical and thermal hyperalgesia, as well as cold allodynia, were reduced in the first hours of treatment. The levels of glutamate and the damage by membrane lipoperoxidation were shown to be improved, and genotoxicity was not demonstrated. In a scenario of therapeutical failures in the treatment of this type of pain, CTK 01512-2 was shown as a new effective alternative protocol. However, further testing is required to determine pharmacokinetics.

中文翻译：

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源自黑夜蛾蜘蛛毒液的重组肽可减轻神经传入障碍引起的伤害感受

摩托车事故中常见的臂丛神经根撕脱是由于传入神经障碍引起的一种神经病。这种类型的疼痛在临床上具有挑战性，因为治疗方案失败或具有严重的副作用。因此，建议评估源自黑夜蛾蜘蛛毒液的重组 CTK 01512-2 肽的镇痛活性，作为未来的新治疗选择。在雄性 Wistar 大鼠组中，通过撕脱上臂丛神经干通过手术诱导神经性疼痛，17 天后，用吗啡、齐考诺肽和 CTK 01512-2 鞘内注射治疗。进行行为测试以评估机械和热痛觉过敏、冷异常性疼痛、前爪的功能活动、和治疗后的探索性运动。治疗后6小时收集外周血样本并进行彗星试验​​。取出脊髓用于膜的脂质过氧化给药。分析脑脊液的谷氨酸剂量。与其他药物相比，重组肽显示出镇痛作用，而不会影响动物的运动活动。在治疗的最初几个小时内，机械和热痛觉过敏以及冷异常性疼痛都减少了。谷氨酸水平和膜脂质过氧化造成的损伤显示出改善，并且没有证明遗传毒性。在治疗此类疼痛的治疗失败的情况下，CTK 01512-2 被证明是一种新的有效替代方案。然而，