In this paper, petaling hydroxyapatite (HA)/TiO₂ composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO₂ surfaces by dip-coating method to endow Ti with good antibacterial and biological properties. The bonding strength between coatings was studied by scratch method. The degradability of CS, BMP-2 release behavior, bioactivity, biocompatibility and antibacterial activity of the obtained (BMP-2)/CS/HA/TiO₂ coatings were examined by in vitro tests. The results showed that, the thicker the HA layer, the larger the loaded BMP-2 and CS amount, resulting in better bonding strength between coatings, antibacterial activity and biocompatibility. In addition, with the increase of CS concentration, more CS was loaded on HA coatings, which benefited the increase of CS degrading amount, the prolonged CS degradation time and BMP-2 release time, resulting in improved antibacterial and biological property. All CS/HA/TiO₂ coatings accelerated cell adhesion, spreading and proliferation, and promoted HA formation in simulated body fluids (SBF). After loading BMP-2 in CS, the BMP-2 can significantly improve cell adhesion, spreading and proliferation, and the loaded amount can also be controlled by the concentration of BMP-2 solution. The present study indicates that, by controlling the thickness of HA layers and concentrations of CS and BMP-2 solutions, the Ti implant material with excellent biological and antibacterial properties can be achieved.

中文翻译：

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在钛表面上制备BMP-2 /壳聚糖/羟基磷灰石抗菌生物复合涂料，用于骨组织工程。

本文首先通过一步微弧氧化（MAO）技术在钛（Ti）表面制备花瓣状羟基磷灰石（HA）/ TiO ₂复合涂层，然后制备纯壳聚糖（CS）和骨形态发生蛋白2（BMP）。通过浸涂法将-2）包封的CS涂层分别负载在HA / TiO ₂表面上，以赋予Ti良好的抗菌和生物学性能。通过刮擦法研究了涂层之间的结合强度。通过体外检测获得的（BMP-2）/ CS / HA / TiO ₂涂层的CS降解性，BMP-2释放行为，生物活性，生物相容性和抗菌活性。测试。结果表明，HA层越厚，BMP-2和CS的负载量越大，涂层之间的结合强度，抗菌活性和生物相容性越好。另外，随着CS浓度的增加，更多的CS负载在HA涂料上，这有利于CS降解量的增加，CS降解时间的延长和BMP-2释放时间的增加，从而提高了抗菌和生物学性能。所有CS / HA / TiO ₂涂层可加速细胞黏附，扩散和增殖，并促进模拟体液（SBF）中HA的形成。在CS中加载BMP-2后，BMP-2可以显着改善细胞粘附，扩散和增殖，并且加载量也可以通过BMP-2溶液的浓度来控制。本研究表明，通过控制HA层的厚度以及CS和BMP-2溶液的浓度，可以获得具有优异生物学和抗菌性能的Ti植入材料。