In the present study significantly increased lipid peroxidation value (LPP) after a single intraperitioneal injection of lead acetate (LA) (100 mg/kg b.w.) indicated enormous generation of Reactive Oxygen Species (ROS). Lead-induced ROS has a direct inhibitory effect on the growth and differentiation of the spermatogonial cells showing a significant decline in sperm count. Chromosomal analysis of the primary spermatocytes at week 4 post-treatment in lead-treated mice revealed significantly higher no of aberrant cells including chromosomal deficiency, autosomal and XY-asynapsis plates compared to untreated control mice, Sperm morphology studies at week 1-4 and at week 8 post-treatment, indicated higher percentage of deformed sperm population compared to vehicle injected groups of mice. Supplementation of vitamin C (Vit C) at a dose of 10 mg/kg body weight to lead-treated mice groups, however, significantly reduced the LPP with a concomitant increase in sperm count, marked decrease in the no of aberrant cells and significant decline in the percentage of morphologically abnormal sperm population. Protective role of Vit C in combating lead-induced oxidative stress in mice testicular cells, has been discussed.

中文翻译：

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维生素C在醋酸铅诱导的瑞士小鼠精子发生中的作用。

在本研究中，一次腹膜内注射乙酸铅（LA）（100 mg / kg bw）后，脂质过氧化值（LPP）显着增加，表明产生了大量的活性氧（ROS）。铅诱导的ROS对精原细胞的生长和分化具有直接抑制作用，显示精子数量显着下降。铅处理的小鼠在治疗后第4周对原代精母细胞的染色体分析显示，与未处理的对照小鼠相比，包括染色体缺乏，常染色体和XY突触板的异常细胞均没有明显增加，在第1-4周和治疗后第8周，表明与载体注射的小鼠组相比，精子畸形的百分比更高。铅治疗的小鼠组补充维生素C（Vit C）剂量为10 mg / kg体重，可显着降低LPP，并伴有精子数量增加，异常细胞数量明显减少和显着下降形态异常的精子群体的百分比。Vit C在对抗小鼠睾丸细胞中铅诱导的氧化应激中的保护作用已被讨论。