Gel filtration chromatography was performed on cytosol preparation of hen spinal cord to find molecular target(s) for organophosphorus-induced delayed neurotoxicity (OPIDN). Three binding peaks of [(3)H]diisopropyl phosphorofluoridate (DFP), an organophosphate that induces OPIDN, were separated from the cytosol preparation. The activities of acetylcholinesterase (AChE) and neuropathy target esterase (NTE) that has been proposed as a screening method for OPIDN eluted in the fractions within these two DFP binding peaks. However, the other peak had none of the activities of AChE and NTE. Therefore, this DFP binding proteins in cytosol may be peculiar to the pathogenesis of OPIDN.

中文翻译：

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脊髓胞质溶胶中磷酸二异丙酯的结合位点的表征。

对鸡脊髓的细胞质制剂进行凝胶过滤色谱法，以发现有机磷诱导的延迟神经毒性（OPIDN）的分子靶标。从细胞溶胶制剂中分离出[（3）H]氟磷酸二异丙基酯（DFP）（诱导OPIDN的有机磷酸酯）的三个结合峰。乙酰胆碱酯酶（AChE）和神经病靶标酯酶（NTE）的活性已被提出作为OPIDN的筛选方法，并在这两个DFP结合峰内的级分中洗脱出来。但是，另一个峰没有AChE和NTE的活性。因此，这种DFP结合蛋白在细胞质中可能是OPIDN的发病机制所特有的。