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A novel human DnaJ protein, hTid-1, a homolog of the Drosophila tumor suppressor protein Tid56, can interact with the human papillomavirus type 16 E7 oncoprotein.
Virology ( IF 3.7 ) Pub Date : 1998-07-31 , DOI: 10.1006/viro.1998.9220
B Schilling 1 , T De-Medina , J Syken , M Vidal , K Münger
Affiliation  

We have cloned hTid-1, a human homolog of the Drosophila tumor suppressor protein Tid56, by virtue of its ability to form complexes with the human papillomavirus E7 oncoprotein. The carboxyl terminal cysteine-rich metal binding domain of E7 is the major determinant for interaction with hTid-1. The carboxyl terminus of E7 is essential for the functional and structural integrity of E7 and has previously been shown to function as a multimerization domain. The hTid-1 protein is a member of the DnaJ-family of chaperones. Its mRNA is widely expressed in human tissues, including the HPV-18-positive cervical carcinoma cell line HeLa and human genital keratinocytes, the normal host cells of the HPVs. The hTid-1 gene has been mapped to the short arm of chromosome 16. The large tumor antigens of polyomaviruses encode functional J-domains that are important for viral replication as well as cellular transformation. The ability of HPV E7 to interact with a cellular DnaJ protein suggests that these two viral oncoproteins may target common regulatory pathways through J-domains.

中文翻译:

一种新型的人类DnaJ蛋白hTid-1(果蝇肿瘤抑制蛋白Tid56的同系物)可以与人类乳头瘤病毒16型E7癌蛋白相互作用。

我们已经克隆了hTid-1,它是果蝇肿瘤抑制蛋白Tid56的人类同源物,因为它具有与人乳头瘤病毒E7癌蛋白形成复合物的能力。E7的富含羧基末端半胱氨酸的金属结合域是与hTid-1相互作用的主要决定因素。E7的羧基末端对于E7的功能和结构完整性是必不可少的,以前已证明它可以充当多聚化结构域。hTid-1蛋白是分子伴侣DnaJ家族的成员。它的mRNA在人类组织中广泛表达,包括HPV-18阳性宫颈癌细胞系HeLa和人类生殖器角质形成细胞(HPV的正常宿主细胞)。hTid-1基因已被定位到16号染色体的短臂上。多瘤病毒的大肿瘤抗原编码功能性J结构域,这对病毒复制以及细胞转化都很重要。HPV E7与细胞DnaJ蛋白相互作用的能力表明,这两种病毒癌蛋白可能通过J结构域靶向共同的调控途径。
更新日期:2019-11-01
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