The green alga, Caulerpa lentillifera, is composed of a single cell with multiple nuclei, but it possesses structures analogous to leaves or fronds, stems or stolons, and roots or rhizoids. To understand molecular mechanisms involved in formation and function of these structures, we carried out RNA-seq analysis of fronds and stolons (including rhizoids). Taking advantage of the decoded genome of C. lentillifera, the present RNA-seq analysis addressed transcripts corresponding to 9,311 genes identified in the genome. RNA-seq data suggested that 8,734 genes are expressed in sporophytes. Despite the siphonous body of the alga, differential gene expression was evident in the two structures. 1,027 (11.8%) and 1,129 (12.9%) genes were preferentially expressed in fronds and stolons, respectively, while the remaining 6,578 (75.3%) genes were expressed at the same level in both. Most genes preferentially expressed in fronds are associated with photosynthesis and plant hormone pathways, including abscisic acid signaling. In contrast, those preferentially expressed in stolons are associated with translation and DNA replication. These results indicate that gene expression is regulated differently between fronds and stolons, which probably governs the function of each structure. Together with genomic information, the present transcriptomic data provide genic information about development and physiology of this unique, siphonous organism.

中文翻译：

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虹吸巨藻Caulerpa lentillifera的叶和茎中的差异基因表达。

绿藻类植物长刺菜刺藻（Caulerpa lentillifera）由具有多个细胞核的单个细胞组成，但其结构类似于叶片或叶状体，茎或茎以及根或根状茎。为了了解涉及这些结构的形成和功能的分子机制，我们进行了叶状和茎（包括根状茎）的RNA序列分析。利用解码后的扁豆梭菌基因组，本RNA-seq分析解决了与基因组中鉴定出的9,311个基因相对应的转录本。RNA-seq数据表明，在孢子体中表达了8,734个基因。尽管藻类呈虹吸状，但在这两个结构中明显存在差异基因表达。1,027（11.8％）和1,129（12.9％）个基因分别在叶状体和茎中优先表达，而其余的6,578（75。两者中有3％的基因以相同的水平表达。大多数在叶中优先表达的基因与光合作用和植物激素途径有关，包括脱落酸信号传导。相比之下，那些优先以脚表达的蛋白与翻译和DNA复制相关。这些结果表明，在叶状和and茎之间基因表达受到不同的调节，这可能决定着每个结构的功能。连同基因组信息，当前的转录组数据提供了有关这种独特的虹吸生物的发育和生理的遗传信息。优先表达在茎中的那些与翻译和DNA复制有关。这些结果表明，在叶状和and茎之间基因表达受到不同的调节，这可能决定着每个结构的功能。连同基因组信息，当前的转录组数据提供了有关这种独特的虹吸生物的发育和生理的遗传信息。优先表达在茎中的那些与翻译和DNA复制有关。这些结果表明，在叶状和and茎之间基因表达受到不同的调节，这可能决定着每个结构的功能。连同基因组信息，当前的转录组数据提供了有关这种独特的虹吸生物的发育和生理的遗传信息。