The expression levels of immunoglobulin elements and their receptors are important markers for health and disease. Within the immunoglobulin locus, the constant regions and the variable region families are associated with certain pathologies, yet a holistic view of the interaction between the expressions of the multiple genes remain to be fully characterized. There is thus an important need to quantify antibody elements, their receptors and the receptor subunits in blood (PBMC cDNA) for both screening and detailed studies of such associations. Leveraging on qPCR, we designed primers for all Vκ1-6, VH1-7, Vλ1-11, nine CH isotypes, Cκ, Cκ, Cλ1 &3, FcεRI α,β, and γ subunits, all three FcγR and their subunits, and FcαR. Validating this on a volunteer PBMC cDNA, we report a qPCR primer set repertoire that can quantify the relative expression of all the above genes to the GAPDH housekeeping gene, with implications and uses in both clinical monitoring and research.

中文翻译：

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使用qPCR定量抗体元件和受体亚基的表达：VH，VL，CH，CL，FcR亚基引物的设计可更全面地了解免疫系统。

免疫球蛋白元素及其受体的表达水平是健康和疾病的重要标志。在免疫球蛋白基因座内，恒定区和可变区家族与某些病理学有关，但是关于多个基因表达之间相互作用的整体观点仍有待充分表征。因此，非常需要定量抗体成分，它们的受体和血液中的受体亚单位（PBMC cDNA），以用于筛选和详细研究这种关联。利用qPCR，我们为所有Vκ1-6，VH1-7，Vλ1-11，九种CH同种型，Cκ，Cκ，Cλ1＆3，FcεRIα，β和γ亚基，所有三个FcγR及其亚基和FcαR设计了引物。在志愿者PBMC cDNA上对此进行验证，