A new bacteriocin produced by Bacillus thuringiensis subsp. entomocidus was identified. The antibacterial activity termed entomocin 110 was produced starting at mid-logarithmic growth phase, reaching its maximum at the early and during stationary phase. The bacteriocin obtained from culture supernatant was inhibitory to several Gram-positive bacteria including Listeria monocytogenes, Paenibacillus larvae and other Bacillus species. Entomocin 110 was shown to be heat stable and resistant to pH variation and to organic solvents. The inhibitory activity was totally lost after proteinase K treatment, thereby revealing its proteinaceous nature. The mode of action of entomocin 110 was bactericidal and bacteriolytic. Upon partial purification with ammonium sulphate precipitation followed by butanol extraction, an active peptide with an apparent molecular weight of 4.8 kDa was identified. Cross inhibition tests with bacteriocin producer strains and plasmid profiles indicated that entomocin 110 is a new bacteriocin, which genetic determinants are probably harbored by the chromosome.

中文翻译：

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新近鉴定出的苏云金芽孢杆菌亚种细菌素entomocin 110的表征和部分纯化。昆虫纲HD110。

苏云金芽孢杆菌亚种产生的一种新的细菌素。鉴定了昆虫。在对数生长期的中期开始产生称为Entomocin 110的抗菌活性，在早期和固定阶段达到最大。从培养上清液中获得的细菌素可抑制几种革兰氏阳性细菌，包括单核细胞增生性李斯特菌，幼芽孢杆菌幼虫和其他芽孢杆菌。Entomocin 110具有热稳定性，并能抵抗pH值变化和有机溶剂。蛋白酶K处理后，抑制活性完全丧失，从而揭示其蛋白质性质。Entomocin 110的作用方式是杀菌和溶菌的。用硫酸铵沉淀部分纯化后，用丁醇萃取，鉴定出具有4.8kDa表观分子量的活性肽。用细菌素生产菌株和质粒图谱进行的交叉抑制测试表明，entomocin 110是一种新的细菌素，其遗传决定因素可能在染色体上。