Hydrogenases are metalloproteins that catalyze the oxidation and reduction of molecular hydrogen and play a crucial role in many microbial metabolic processes. A subset of hydrogenases capable of functioning at temperatures from 50 to 125 degrees C is found in thermophilic microorganisms. Most known thermotolerant hydrogenases contain a [NiFe] active site and are either bidirectional or uptake type. Although no exhaustive survey has been done of the ecological diversity of thermophilic hydrogen-reducing or oxidizing bacteria, they appear to exist in virtually every thermophilic environment examined to date. Thermotolerant hydrogenases share many similarities with their mesophilic counterparts, but they have several features in addition to thermotolerance that make them especially well suited for biotechnological applications. Ongoing research is focused on potential applications of thermotolerant H2 ases in biosynthesis, H2 production, bioremediation, and biosensors.

中文翻译：

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耐热氢酶：生物多样性，特性和生物技术应用。

氢化酶是金属蛋白，可催化分子氢的氧化和还原，并在许多微生物代谢过程中发挥关键作用。在嗜热微生物中发现了能够在50到125摄氏度的温度下起作用的氢化酶的子集。最已知的耐热性氢酶包含[NiFe]活性位点，是双向或摄取型。尽管尚未对嗜热氢还原或氧化细菌的生态多样性进行详尽的调查，但它们似乎存在于迄今为止研究的几乎每个嗜热环境中。耐热性氢酶与其嗜温性对应物有许多相似之处，但是它们除了具有耐热性外还具有其他功能，这使其特别适合于生物技术应用。