Background: During storage of erythrocytes, storage lesions are formed that reduce the safety and efficacy of the stored blood. Thus, there is a need to understand the changes that occur during storage. Most studies have focused on storage of a mixed population of erythrocytes. The aim of this study is to analyze the changes in young and old erythrocytes over the course if storage. Materials and Methods: Blood was collected from the blood bank at the Kempegowda Institute of Medical Sciences (KIMS) Hospital (Bengaluru, India) and stored for 35 days in CPDA-1 at 4°C. Every 5 days, erythrocytes were separated based on the blood’s age using a Percoll-BSA gradient. Young and old erythrocytes obtained were used for analysis of the following oxidative stress (OS) markers: hemoglobin (Hb), hemolysis, mechanical fragility, antioxidant enzymes (superoxide dismutase and catalase [CAT]), superoxides, sialic acid, glutamic oxaloacetate transaminase (GOT), glucose, plasma membrane redox system (PMRS), total antioxidant capacity-cupric ion reducing antioxidant capacity assay (TACCUPRAC), lactate dehydrogenase (LDH), lipid peroxidation products (malondialdehyde [MDA] and conjugate dienes), and protein oxidation products (advanced oxidation protein products and protein sulfhydryls). Result: Young cells had higher amounts of Hb, sialic acid, GOT, LDH, TACCUPRAC, CAT, and superoxides compared to old cells. Old cells, however, had higher PMRS and MDA levels with respect to young cells. Discussion: Young cells could endure OS during storage more efficiently than old cells. In conclusion, the efficacy of stored blood depends on the ratio of young cells in the population. This study highlights the prospects of storing young erythrocytes for a prolonged shelf life of blood.

中文翻译：

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血库条件下红细胞的年龄相关调节

背景：红细胞在储存过程中会形成储存损伤，降低储存血液的安全性和有效性。因此，需要了解存储过程中发生的变化。大多数研究都集中在混合红细胞群的储存上。本研究的目的是分析年轻和年老红细胞在储存过程中的变化。材料和方法：血液采集自 Kempegowda 医学科学研究所 (KIMS) 医院（印度班加罗尔）的血库，并在 4°C 的 CPDA-1 中储存 35 天。每 5 天，使用 Percoll-BSA 梯度根据血液的年龄分离红细胞。获得的年轻和年老红细胞用于分析以下氧化应激 (OS) 标记：血红蛋白 (Hb)、溶血、机械脆性、抗氧化酶（超氧化物歧化酶和过氧化氢酶 [CAT]）、超氧化物、唾液酸、谷氨酸草酰乙酸转氨酶 (GOT)、葡萄糖、质膜氧化还原系统 (PMRS)、总抗氧化能力-铜离子还原抗氧化能力测定 (TACCUPRAC)、乳酸脱氢酶(LDH)、脂质过氧化产物（丙二醛 [MDA] 和共轭二烯）和蛋白质氧化产物（高级氧化蛋白质产物和蛋白质巯基）。结果：与老细胞相比，年轻细胞具有更高量的 Hb、唾液酸、GOT、LDH、TACCUPRAC、CAT 和超氧化物。然而，相对于年轻细胞，老细胞具有更高的 PMRS 和 MDA 水平。讨论：年轻细胞在储存期间比老细胞更有效地耐受 OS。总之，储存血液的功效取决于种群中年轻细胞的比例。