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Transpososome dynamics and regulation in Tn10 transposition.
Critical Reviews in Biochemistry and Molecular Biology ( IF 6.5 ) Pub Date : 2006-11-10 , DOI: 10.1080/10409230600987415
David B Haniford 1
Affiliation  

Tn10 is a bacterial transposon that transposes through a non-replicative mechanism. This mode of DNA transposition is widely used in bacteria and is also used by "DNA-based" transposons in eukaryotes. Tn10 has served as a paradigm for this mode of transposition and continues to provide novel insights into how steps in transposition reactions occur and how these steps are regulated. A common feature of transposition reactions is that they require the formation of a higher order protein-DNA complex called a transpososome. A major objective in the last few years has been to better understand the dynamics of transpososome assembly and progression through the course of transposition reactions. This problem is particularly interesting in the Tn10 system because two important host proteins, IHF and H-NS, have been implicated in regulating transpososome assembly and/or function. Interestingly, H-NS is an integral part of stress response pathways in bacteria, and its function is known to be sensitive to changes in environmental conditions. Consequently, H-NS may provide a means of allowing Tn10 to responed to changing environmental conditions. The current review focuses on the roles of both IHF and H-NS on Tn10 transposition.

中文翻译:

Tn10转座中的转座体动力学和调控。

Tn10是通过非复制机制转座的细菌转座子。这种DNA转座模式广泛用于细菌中,也被真核生物中的“基于DNA的”转座子使用。Tn10已成为这种转座模式的范例,并继续提供有关转座反应步骤如何发生以及如何调节这些步骤的新颖见解。转座反应的共同特征是它们需要形成称为转座体的高级蛋白质-DNA复合体。过去几年的主要目标是通过转座反应的过程更好地了解转座体组装和进展的动力学。这个问题在Tn10系统中特别有趣,因为两个重要的宿主蛋白IHF和H-NS 已经涉及调节转座体的装配和/或功能。有趣的是,H-NS是细菌应激反应途径不可或缺的一部分,已知其功能对环境条件的变化敏感。因此,H-NS可提供允许Tn10响应不断变化的环境条件的手段。目前的审查侧重于IHF和H-NS在Tn10换位上的作用。
更新日期:2019-11-01
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