Semisynthetic cephalosporins are important antibacterials in clinical practice. Semisynthetic cephalosporins are manufactured by derivatizing 7-aminocephalosporanic acid (7-ACA) and its desacetylated form. Microbial enzymes such as D-amino acid oxidase, glutaryl-7-ACA acylase and cephalosporin esterase are being used as biocatalysts for the conversion of cephalosporin C (CEPH-C) to 7-ACA and its desacetylated derivatives. Recent developments in the field of enzymatic modifications of cephalosporin with special emphasis on group of enzymes called as cephalosporin acylase is discussed in this review. Aspects related to screening methods, isolation and purification, immobilization, molecular cloning, gene structure and expression and protein engineering of cephalosporin acylases have been covered. Topics pertaining to enzymatic modifications of cephalosporin by D-amino acid oxidase, cephalosporin methoxylase and beta-lactamase are also covered.

中文翻译：

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头孢菌素酰基转移酶和其他酶对头孢菌素的酶修饰。

在临床实践中，半合成头孢菌素是重要的抗菌剂。半合成头孢菌素是通过衍生化7-氨基头孢烷酸（7-ACA）及其去乙酰化形式而制得的。微生物酶，例如D-氨基酸氧化酶，戊二酰7-ACA酰基转移酶和头孢菌素酯酶，被用作将头孢菌素C（CEPH-C）转化为7-ACA及其脱乙酰基衍生物的生物催化剂。在这篇综述中讨论了头孢菌素的酶促修饰领域的最新进展，其中特别强调了称为头孢菌素酰基转移酶的一组酶。涵盖了与头孢菌素酰基转移酶的筛选方法，分离和纯化，固定化，分子克隆，基因结构和表达以及蛋白质工程有关的方面。