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Growth and siderophore production of Xylella fastidiosa under iron-limited conditions.
Microbiological Research ( IF 6.7 ) Pub Date : 2005-11-01 , DOI: 10.1016/j.micres.2005.03.007
Maria Estela Silva-Stenico 1 , Flávia Tereza Hansen Pacheco , Jorge Luiz Mazza Rodrigues , Emanuel Carrilho , Siu Mui Tsai
Affiliation  

In this study, the production of siderophores by Xylella fastidiosa from the citrus bacteria isolate 31b9a5c (FAPESP - ONSA, Brazil) was investigated. The preliminary evidence supporting the existence of siderophore in X. fastidiosa was found during the evaluation of sequencing data generated in our lab using the BLAST-X tool, which indicated putative open reading frames (ORFs) associated with iron-binding proteins. In an iron-limited medium siderophores were detected in the supernatant of X. fastidiosa cultures. The endophytic bacterium Methylobacterium extorquens was also evaluated. Capillary electrophoresis was used to separate putative siderophores produced by X. fastidiosa. The bacterial culture supernatants of X. fastidiosa were identified negative for hydroxamate and catechol and positive for M. extorquens that secreted hydroxamate-type siderophores.

中文翻译:

铁限制条件下小木球藻的生长和铁载体的产生。

在这项研究中,研究了由木霉菌分离株31b9a5c(FAPESP-ONSA,巴西)生产的木霉菌产生的铁载体。在对我们实验室中使用BLAST-X工具生成的测序数据进行评估的过程中,发现了支持X. fastidiosa中铁载体存在的初步证据,这表明与铁结合蛋白相关的推定开放阅读框(ORF)。在铁有限的培养基中,在X.fastidiosa培养物的上清液中检测到铁载体。还评估了内生细菌嗜甲基杆菌。毛细管电泳被用来分离假单胞菌产生的假定的铁载体。鉴定到的X.fastidiosa细菌培养上清液对异羟肟酸酯和儿茶酚呈阴性,对M呈阳性。
更新日期:2019-11-01
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