Multiple shoots were induced from leaf explants of Lycopersicon esculentum cultivar MicroTom, within 20-25d, on MS medium supplemented with 8.9 microM benzylaminopurine (BAP)+1.14 microM indole-3-acetic acid (IAA). For rooting, elongated microshoots were excised and transferred onto MS medium supplemented with 4.9 microM indole-3-butyric acid (IBA). Well-developed roots and flower raceme were obtained on d 7 and 13, respectively, upon transfer of the microshoots onto rooting medium. The flowers self-fertilized in vitro and produced mature fruits in additional 15-17d of culture.

中文翻译：

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从叶段诱导多芽，矮番茄（Lycopersicon esculentum）的体外开花和结果

从番茄栽培品种 MicroTom 的叶外植体中诱导多芽，在 20-25 d 内，在补充有 8.9 microM 苄基氨基嘌呤 (BAP) + 1.14 microM 吲哚-3-乙酸 (IAA) 的 MS 培养基上。为了生根，切出细长的微芽并将其转移到补充有 4.9 microM 吲哚-3-丁酸 (IBA) 的 MS 培养基上。将微芽转移到生根培养基上后，分别在第 7 天和第 13 天获得发育良好的根和总状花序。花在体外自体受精，并在额外的 15-17 天培养中产生成熟的果实。