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Improving -tocopherol production in plant cell cultures
Journal of Plant Physiology ( IF 4.3 ) Pub Date : 2005-07-01 , DOI: 10.1016/j.jplph.2005.04.010
Rosa Gala 1 , Giovanni Mita , Sofia Caretto
Affiliation  

Suspension cell cultures of Helianthus annuus L. were previously established for the production of the most active component of vitamin E, alpha-tocopherol, by optimizing medium composition and culture conditions. In the present work, the possibility of enhancing alpha-tocopherol production by the addition of jasmonic acid to the culture medium was investigated both in sunflower and Arabidopsis cell cultures. A considerable increase (49% and 66%, respectively) of alpha-tocopherol production was obtained in both, after a 72-h treatment with 5 microM jasmonic acid. The modulation of alpha-tocopherol levels in plant cell cultures can provide useful hints for a regulatory impact on tocopherol metabolism.

中文翻译:

提高植物细胞培养中生育酚的产量

以前建立了向日葵悬浮细胞培养物,通过优化培养基组成和培养条件来生产维生素 E 中最活跃的成分 α-生育酚。在目前的工作中,在向日葵和拟南芥细胞培养物中研究了通过向培养基中添加茉莉酸来提高 α-生育酚产量的可能性。用 5 microM 茉莉酸处理 72 小时后,两者的 α-生育酚产量均显着增加(分别为 49%和 66%)。植物细胞培养物中 α-生育酚水平的调节可以为对生育酚代谢的调节影响提供有用的提示。
更新日期:2005-07-01
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