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Chlorophyll accumulation is enhanced by osmotic stress in graminaceous chlorophyllic cells
Journal of Plant Physiology ( IF 4.3 ) Pub Date : 2005-06-01 , DOI: 10.1016/j.jplph.2004.09.015
Xóchitl García-Valenzuela 1 , Edmundo Garcá-Moya , Quintín Rascón-Cruz , Luis Herrera-Estrella , Gerardo Armando Aguado-Santacruz
Affiliation  

We have developed a new chlorophyllic cell line ('TADH-XO') from the highly water stress tolerant grass Bouteloua gracilis (blue grama). When grown under normal (non-stress) conditions, this new cell line accumulates higher levels of chlorophyll (up to 368.1 microg total chlorophyll g(-1) FW) than a previously obtained cell line ('TIANSJ98'). Both cell lines are capable of developing substantially higher amounts of chlorophyll when subjected to osmotic stress. In order to explain these changes in the chlorophyll kinetics of the chlorophyllic cells, we analyzed the following population variables in cells subjected to polyethylene glycol 8000-induced osmotic stress: growth, viability, chlorophyll (total, 'a' and 'b'), cell size, percentage of green cells and chloroplast (number and size). Although previous studies in some chlorophyllic cells of dicots have already reported that chlorophyll increases under saline stress, in this report we show that, at least in this graminaceous cell line, the increase in chlorophyll is an immediate and proportional response to the osmotic stress applied and not the result of a progressive adaptation process. Consistent with previous studies, the increase in chlorophyll accumulation could be the result of chloroplast development (increased thylakoid number per chloroplast). On the basis of our results, the increases in chlorophyll accumulation previously observed in salt-adapted dicot cells may be the result of the osmotic shock (water deficit), rather than the ionic effect of salt on the physiology of chlorophyllic cells of dicots. Under the cell population experimental approach we followed, our study provides important insights related to the physiological behavior of chlorophyllic cells subjected to osmotic stress.

中文翻译:

禾本科叶绿素细胞中的渗透胁迫增强了叶绿素的积累

我们开发了一种新的叶绿素细胞系 ('TADH-XO'),来自高度耐水胁迫的草 Buteloua gracilis (blue grama)。当在正常(无压力)条件下生长时,这种新细胞系比以前获得的细胞系 ('TIANSJ98') 积累更高水平的叶绿素(高达 368.1 microg 总叶绿素 g(-1) FW)。当受到渗透胁迫时,两种细胞系都能够产生显着更高量的叶绿素。为了解释叶绿素细胞叶绿素动力学的这些变化,我们分析了受到聚乙二醇 8000 诱导的渗透胁迫的细胞中的以下种群变量:生长、活力、叶绿素(总计、“a”和“b”)、细胞大小、绿色细胞和叶绿体的百分比(数量和大小)。虽然之前对双子叶植物的一些叶绿素细胞的研究已经报道了在盐胁迫下叶绿素增加,但在本报告中,我们表明,至少在这种禾本科细胞系中,叶绿素的增加是对所施加的渗透胁迫的直接和成比例的反应。不是渐进适应过程的结果。与之前的研究一致,叶绿素积累的增加可能是叶绿体发育的结果(每个叶绿体的类囊体数量增加)。根据我们的结果,先前在盐适应双子叶植物细胞中观察到的叶绿素积累增加可能是渗透休克(水分不足)的结果,而不是盐对双子叶植物叶绿素细胞生理的离子效应。在我们遵循的细胞群实验方法下,
更新日期:2005-06-01
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