Tendon and ligament shows extremely limited endogenous regenerative capacity. Current treatments are based on the replacement and or augmentation of the injured tissue but the repaired tissue rarely achieve functionality equal to that of the preinjured tissue. To address this challenge, tissue engineering has emerged as a promising strategy. This study develops a regenerative and resorbable hybrid construct for tendon and ligament engineering. The construct is made up by a hollow poly-lactic acid braid with embedded microspheres carrying cells and an anti-adherent coating, with all the parts being made of biodegradable materials. This assembly intends to regenerate the tissue starting from the interior of the construct towards outside while it degrades. Fibroblasts cultured on poly lactic acid and hyaluronic acid microspheres for 6 h were injected into the hollow braid and the construct was cultured for 14 days. The cells thus transported into the lumen of the construct were able to migrate and adhere to the braid fibers naturally, leading to a homogeneous proliferation inside the braid. Moreover, no cells were found on the outer surface of the coating. Altogether, this study demonstrated that PLA/HA hybrid construct could be a promising material for tendon and ligament repair.

中文翻译：

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用于肌腱/韧带组织工程的可再生和可吸收的PLA / HA杂化构建体。

肌腱和韧带显示出极其有限的内生再生能力。当前的治疗是基于受伤组织的替换和/或增加，但是修复后的组织很少能达到与受伤前组织相同的功能。为了应对这一挑战，组织工程学已成为一种有前途的策略。这项研究开发了一种用于肌腱和韧带工程的可再生和可吸收的混合构建体。该结构由空心聚乳酸编织物组成，该编织物带有嵌入的携带细胞的微球和抗粘附涂层，所有部件均由可生物降解的材料制成。该组件旨在在组织降解的同时从构建体内部向外部再生组织。将在聚乳酸和透明质酸微球上培养6 h的成纤维细胞注入空心编织物中，并将构建体培养14天。如此转运到构建体腔中的细胞能够自然迁移并粘附到编织纤维上，从而导致编织物中的均匀增殖。此外，在涂层的外表面上未发现细胞。总而言之，这项研究表明PLA / HA杂合体构建体可能是腱和韧带修复的有前途的材料。