Bacterial oligopeptide transporters encoded by arrays of opp genes are implicated in a wide variety of physiological functions including nutrient acquisition, cell-to-cell communication, host-pathogen interaction. Combining the five opp genes in one oppABCDF operon of Escherichia coli assumes unified principle of their transcriptional regulation, which should provide a comparable amounts of translated products. This, however, contradicts the experimentally detected disproportion in the abundance of periplasmic OppA and the trans-membrane subunits OppB and OppC. As a first step towards understanding differential regulation of intraoperonic genes we examined genomic region proximal to oppB for its competence to initiate RNA synthesis using in silico promoter predictions, data of high-throughput RNA sequencing and targeted transcription assay. A number of transcription start sites (TSSs), whose potency depends on the presence of cationic oligopeptide protamine in cultivation medium, was found at the end of oppA and in the early coding part of oppB. We also show that full-size OppB conjugated with EGFP is produced under the control of its own genomic regulatory region and may be detected in analytical quantities of bacterial cell culture.

中文翻译：

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在计算预测和高通量转录谱分析之间：大肠杆菌OppABCDF寡肽转运蛋白OppB跨膜亚基的深度表达分析。

由opp基因阵列编码的细菌寡肽转运蛋白与多种生理功能有关，包括营养获取，细胞间通讯，宿主-病原体相互作用。将五个opp基因组合到大肠杆菌的一个oppABCDF操纵子中，假定它们具有统一的转录调节原理，这应提供相当数量的翻译产物。但是，这与实验检测到的在周质OppA和跨膜亚基OppB和OppC丰度上的不相符。作为理解差动内基因差异调节的第一步，我们使用计算机启动子预测，高通量RNA测序数据和靶向转录分析，研究了oppB附近的基因组区域启动RNA合成的能力。在oppA的末尾和oppB的早期编码部分发现了许多转录起始位点（TSS），其效力取决于培养基中阳离子寡肽鱼精蛋白的存在。我们还显示，与EGFP偶联的全尺寸OppB是在其自身的基因组调控区的控制下产生的，并且可以在分析量的细菌细胞培养物中检测到。