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Nitric oxide-loaded chitosan nanoparticles as an innovative antileishmanial platform.
Nitric Oxide ( IF 3.9 ) Pub Date : 2019-09-18 , DOI: 10.1016/j.niox.2019.09.007
Fernanda V Cabral 1 , Milena T Pelegrino 2 , Ismael P Sauter 3 , Amedea B Seabra 2 , Mauro Cortez 3 , Martha S Ribeiro 1
Affiliation  

Leishmaniasis is a neglected tropical disease that demands for new therapeutic strategies due to adverse side effects and resistance development promoted by current drugs. Nitric oxide (NO)-donors show potential to kill Leishmania spp. but their use is limited because of their instability. In this work, we synthesize, characterize, and encapsulate S-nitroso-mercaptosuccinic acid into chitosan nanoparticles (NONPs) and investigate their activity on promastigotes and intracellular amastigotes of Leishmania (Leishmania) amazonensis. Cytotoxicity on macrophages was also evaluated. We verified that NONPs reduced both forms of the parasite in a single treatment. We also noticed reduction of parasitophorous vacuoles as an evidence of inhibition of parasite growth and resolution of infection. No substantial cytotoxicity was detected on macrophages. NONPs were able to provide a sustained parasite killing for both L. (L.) amazonensis infective stages with no toxicity on macrophages, representing a promising nanoplatform for cutaneous leishmaniasis.

中文翻译:

负载一氧化氮的壳聚糖纳米颗粒作为创新的抗污平台。

利什曼病是一种被忽视的热带病,由于当前药物所引起的不良副作用和耐药性的发展,因此需要新的治疗策略。一氧化氮(NO)供体显示出杀死利什曼原虫的潜力。但由于其不稳定,因此使用受到限制。在这项工作中,我们合成,表征,并将S-亚硝基巯基琥珀酸封装到壳聚糖纳米颗粒(NONPs)中,并研究它们对亚马逊利什曼原虫(Leishmania)亚马逊前鞭毛体和胞内无脊椎动物的活性。还评估了对巨噬细胞的细胞毒性。我们验证了NONP在一次治疗中可同时减少两种形式的寄生虫。我们还注意到寄生虫空泡的减少是抑制寄生虫生长和感染消退的证据。在巨噬细胞上未检测到实质性的细胞毒性。
更新日期:2019-11-01
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