Iron uptake system is expressed in early stages of Acinetobacter baumannii infections under iron-restricted conditions. This study is aimed at the evaluation of immuno-protectivity of BfnH in comparison with BauA in both mature and selected fragmental proteins. The study was designed in single and combined forms of antigens. BfnH is presented in 3472 strains of A. baumannii with more than 97% identity. The preliminary immune-informatics analysis of this protein indicated a region from the β-barrel domain including exposed loops 2-5, with antigenic score comparable to that of BfnH. There was a significant rise in the specific IgG response in all test groups. The bacterial challenge with a lethal dose of A. baumannii demonstrated partial protection of whole proteins which coincides with a significant reduction in the bacterial population colonized in the main organs and an increase in the survival level. Passive immunization of the mice brought about 50% survival in the mice groups immunized with BfnH and with a combination of BfnH and BauA. The protectivity of siderophore receptors suggests their potential immunogenic role that could be considered as a component of multivalent subunit vaccine candidates against A. baumannii.

中文翻译：

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利用两种铁载体BauA和BfnH来保护鲍曼不动杆菌感染。

铁限制系统在鲍曼不动杆菌感染的早期表达铁摄取系统。这项研究旨在评估BfnH与BauA在成熟和选定片段蛋白中的免疫保护性。该研究设计为单一和组合形式的抗原。BfnH存在于3472株鲍曼不动杆菌中，具有97％以上的同一性。对该蛋白的初步免疫信息学分析表明，β-桶状结构域的一个区域包括暴露的环2-5，其抗原评分与BfnH相当。在所有测试组中，特异性IgG反应均显着上升。用致死剂量的A进行细菌攻击。鲍曼氏菌显示出对全蛋白的部分保护，这与定植在主要器官中的细菌种群的显着减少以及存活水平的增加相吻合。小鼠的被动免疫在用BfnH以及BfnH和BauA组合免疫的小鼠组中带来了约50％的存活率。铁载体受体的保护性表明它们潜在的免疫原性作用，可以被认为是针对鲍曼不动杆菌的多价亚基候选疫苗的组成部分。