Chikungunya virus (CHIKV) is a mosquito‐borne pathogen that causes CHIKV fever. Definitive diagnosis is crucial for patients experiencing symptoms similar to other arboviral diseases because they can vary in clinical consequences. An increasing number of patients experience long‐term rheumatic effects of CHIKV infection, but these cases may not be optimally detected by molecular assays and anti‐CHIKV IgM ELISAs (M‐ELISAs) used for confirmation and screening, respectively. The subsequent confirmatory serological test, the plaque reduction neutralization test (PRNT), is laborious and time‐consuming. In this study, we evaluated a new diagnostic algorithm in which the M‐ELISA is conducted in parallel with an anti‐CHIKV IgG ELISA (G‐ELISA) and observed that the Euroimmun M‐ELISA combined with the Euroimmun G‐ELISA or the Abcam G‐ELISA exhibited excellent sensitivity and specificity for CHIKV. The combinations demonstrated perfect and near perfect inter‐rater agreement with the PRNT, respectively, suggesting their potential to be used as alternatives to the confirmatory serological PRNT assay for CHIKV.

中文翻译：

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结合抗IgM和IgG免疫测定进行全面的基孔肯雅病毒诊断测试。

基孔肯雅病毒（CHIKV）是由蚊子传播的病原体，可引起CHIKV发热。明确的诊断对于经历与其他虫媒病毒疾病相似的症状的患者至关重要，因为它们的临床后果可能会有所不同。越来越多的患者经历了CHIKV感染的长期风湿病影响，但是这些情况可能无法分别通过用于确认和筛选的分子检测和抗CHIKV IgM ELISA（M-ELISA）进行最佳检测。随后的验证性血清学测试，噬斑减少中和测试（PRNT）既费力又费时。在这个研究中，我们评估了一种新的诊断算法，其中M-ELISA与抗CHIKV IgG ELISA（G-ELISA）并行进行，并观察到Euroimmun M-ELISA与Euroimmun G-ELISA或Abcam G-ELISA结合使用对CHIKV具有极好的敏感性和特异性。两种组合分别显示出与PRNT的完美的和接近完美的评估者之间的一致性，表明它们有可能被用作CHIKV的确证性血清PRNT测定的替代方法。