Currently, adoptive immunotherapy is considered as one of the leading treatments in cancer. Successful adoptive immunotherapy depends on producing large numbers of desired T cells ex vivo for infusion. This requires an effective protocol for maximum functional T-cell expansion while keeping the time and costs to a minimum. Current T-cell expansion protocols are diverse in their methodology, and a universal protocol of expansion is wanting. Also, new findings regarding T-cell biology, signaling, and activation have reshaped the strategies of T-cell propagation over the years, introducing new ways to expand T cells. Here, we reviewed different conditions for blood-derived polyclonal T-cell expansion so as to elucidate the influential factors of T-cell expansion and their efficacy.

中文翻译：

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各种培养条件对过继免疫疗法对多克隆T细胞体外扩增的影响。

当前，过继免疫疗法被认为是癌症的主要疗法之一。成功的过继免疫疗法取决于离体产生大量所需的T细胞进行输注。这需要有效的协议，以最大程度地扩展T细胞功能，同时将时间和成本降至最低。当前的T细胞扩展协议在其方法上是多种多样的，并且需要通用的扩展协议。而且，多年来有关T细胞生物学，信号传导和激活的新发现改变了T细胞繁殖的策略，引入了扩展T细胞的新方法。在这里，我们审查了血液衍生的多克隆T细胞扩增的不同条件，以阐明T细胞扩增的影响因素及其功效。