Hypocotyl segments of 7-day old seedlings of flax (Linum usitatissimum L.) cultivars Atalante, Flanders, Jitka, Szegedi 30 and Super were screened for organogenesis (shoot and root induction) and embryo-like structure production. A non-destructive assay for hydroxyl radicals (*OH), utilising DMSO as a radical trap, was used to determine *OH formation during tissue culture and morphogenesis. Desferrioxamine, an inhibitor of Fenton reaction, and 4-hydroxy-2-nonenal, a cytotoxic Lipid peroxidation product, were exogenously applied to flax cultures to determine the effect of antioxidative and prooxidative status on morphogenetic responses induced through the exogenous application of plant growth regulators. Flax genotypes varied in their response to treatments after exposure to different plant hormones. Hydroxyl radical (*OH) formation correlated with morphogenetic responses and this was affected by plant hormones. Desferrioxamine and 4-hydroxy-2-nonenal also moderated morphogenetic responses and influenced hydroxyl radical formation during in vitro propagation.

中文翻译：

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羟基壬烯醛和去铁胺调节亚麻体外培养物中的形态发生和氧化应激反应

筛选 7 天龄的亚麻 (Linum usitatissimum L.) 栽培品种 Atalante、Flanders、Jitka、Szegedi 30 和 Super 的下胚轴段进行器官发生（芽和根诱导）和胚样结构的产生。使用 DMSO 作为自由基陷阱的羟基自由基 (*OH) 的非破坏性测定用于确定组织培养和形态发生过程中的 *OH 形成。芬顿反应抑制剂去铁胺和细胞毒性脂质过氧化产物 4-羟基-2-壬烯醛外源应用于亚麻培养物，以确定抗氧化和促氧化状态对通过外源应用植物生长调节剂诱导的形态发生反应的影响. 亚麻基因型在暴露于不同的植物激素后对处理的反应各不相同。羟基自由基 (*OH) 的形成与形态发生反应相关，这受植物激素的影响。去铁胺和 4-羟基-2-壬烯醛还可以调节形态发生反应并影响体外繁殖过程中羟基自由基的形成。